Alberta NINT/13 June 2008

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(Difference between revisions)
Line 7: Line 7:
lab work (JD):
lab work (JD):
             Quantitated K102005/BamH1 and Nco1
             Quantitated K102005/BamH1 and Nco1
-
             Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution)
+
             Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution) to create plasmid K102007
-
             Transformed XL1-B cells with ligation and negative control
+
             Transformed XL1-B cells with K102007 and negative control
             Plated cells and left them for O/N
             Plated cells and left them for O/N

Revision as of 17:34, 13 June 2008

lab work (SD): 

            Using the transformation completed by Wayne (12/06/07) of XL1-B cells with I732018 and the overnight
            cultures, DNA was isolated by QIAprep standard miniprep protocol.
            I732018 was sequenced.
            I732018 was digested with XbaI + PstI.

lab work (JD): 

            Quantitated K102005/BamH1 and Nco1
            Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution) to create plasmid K102007
            Transformed XL1-B cells with K102007 and negative control
            Plated cells and left them for O/N
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