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Team:Bologna/Notebook
From 2008.igem.org
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(→Week 2: from 07/28/08 to 08/03/08) |
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| - | + | 1. Plasmid Amplification from 2008 Registry Collection: [http://partsregistry.org/Part:BBa_R0082 R0082,] [http://partsregistry.org/Part:BBa_R0083, R0083], [http://partsregistry.org/wiki/index.php/Part:BBa_M30109 M30109] in TOP10 strain to build and characterize the Light response system to be our spatial selective trigger. <br> | |
| - | + | 2. Plasmid Amplification from 2008 Registry Collection: [http://partsregistry.org/Part:BBa_E0240 E0240], [http://partsregistry.org/Part:BBa_P1010 pSB3K3_P1010] and the Practice Promoter Set ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J23103/ J23150, J23151, J23102]) to test and set up the new [http://partsregistry.org/Measurement Biobrick Standard Measurement Protocol] | |
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| - | + | 3. Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240 <br> | |
= Week 3: from 08/04/08 to 08/10/08 = | = Week 3: from 08/04/08 to 08/10/08 = | ||
Revision as of 13:07, 23 October 2008
| HOME | PROJECT | TEAM | SUBMITTED PARTS | MODELING | WETLAB | LAB-BOOK | BIOSAFETY AND PROTOCOLS |
|---|
Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.
Protocols
| Protocol | Biosafety level |
| Plates preparation | |
| Amplification | |
| Transformation | |
| Inoculation | |
| Miniprep | |
| Digestion reaction | |
| Gel preparation | |
| Electrophoretic run | |
| Gel extraction | |
| Ligation reaction | |
| Chemiocompetent cells | |
| Mediums and buffers | |
| Antibiotics stocks preparation | |
| IPTG stocks preparation | |
| Fluorescence test | |
| M9 supplemented media | |
Week 1: from 07/21/08 to 07/27/08
General Preparations
- Preparation of chemiocompetent cells from E. Coli DH5α, Top10 and DB 3.1
- Preparation of antibiotic stocks for Ampicillin and Kanamicin
- Preparation of LB medium and LB plates for cloning.
Week 2: from 07/28/08 to 08/03/08
1. Plasmid Amplification from 2008 Registry Collection: [http://partsregistry.org/Part:BBa_R0082 R0082,] [http://partsregistry.org/Part:BBa_R0083, R0083], [http://partsregistry.org/wiki/index.php/Part:BBa_M30109 M30109] in TOP10 strain to build and characterize the Light response system to be our spatial selective trigger.
2. Plasmid Amplification from 2008 Registry Collection: [http://partsregistry.org/Part:BBa_E0240 E0240], [http://partsregistry.org/Part:BBa_P1010 pSB3K3_P1010] and the Practice Promoter Set ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J23103/ J23150, J23151, J23102]) to test and set up the new [http://partsregistry.org/Measurement Biobrick Standard Measurement Protocol]
3. Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240
Week 3: from 08/04/08 to 08/10/08
Week 4: from 08/11/08 to 08/17/08
Week 5: from 08/18/08 to 08/24/08
Week 6: from 08/25/08 to 08/31/08
Week 7: from 09/01/08 to 09/07/08
Week 8: from 09/08/08 to 09/14/08
Week 9: from 09/15/08 to 09/21/08
Week 10: from 09/22/08 to 09/28/08
Week 11: from 09/29/08 to 10/05/08
Week 12: from 10/06/08 to 10/12/08
Week 13: from 10/13/08 to 10/19/08
Week 14: from 10/20/08 to 10/26/08
Week 15: from 10/27/08 to 10/29/08
