Team:Imperial College/Summary

From 2008.igem.org

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{{Imperial/StartPage2}}
{{Imperial/StartPage2}}
=== Summer Summary ===
=== Summer Summary ===
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{{Imperial/Box2|Our Approach|[[Image:Cycle.PNG|center|500px]]<br>
 
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The Imperial College 2008 team chose to take the engineering approach to the biofabricator project. This framework has helped us organised the project and team.  |}}
 
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{{Imperial/Box1|Design|
{{Imperial/Box1|Design|
In order to achieve our specifications of design previously described, we require the following devices;  
In order to achieve our specifications of design previously described, we require the following devices;  
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{{Imperial/Box1|Testing|
{{Imperial/Box1|Testing|
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The wet lab team was responsible for designing the constructs and setting up the cloning strategy to get us from the starting parts to the finished system. We were also responsible for designing and BioBricking the starting parts, of course, and designing and implementing the integration brick technique. This section shows some of the major results that came from the wet lab over the summer. Motility results can go in the dry lab overview above.
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The testing and validation of our project can be split into three main areas;
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====== Transformation ======
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*'''Work with ''B.subtilis''''' - Including characterisation of growth curves and transformation,
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Transformation stuff goes here...
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*'''Characterisation and control of motility'''
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====== Calibration Curve ======
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*'''Production of Biomaterials in B.subtilis'''
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Calibration curve results go here...
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|Stuff}}
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Please see the [https://2008.igem.org/Team:Imperial_College/Major_Results Results Page] for more information on the key results from the testing and validation.
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|[[Image:Result.PNG|center|300px]]
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{{Imperial/Box1|Achievements|
{{Imperial/Box1|Achievements|

Revision as of 23:47, 23 October 2008

Summer Summary

Design

In order to achieve our specifications of design previously described, we require the following devices;

  • Light sensing device - Converting a light input into a PoPS output,
  • Biomaterial production device- Converting a PoPS input into an output of biomaterial production,
  • Motility Control device - Converting a PoPS input into an output of motility arrest,
  • Integration device - To allow integration and selection of our genetic constructs and devices into B,subtilis,


Each of these constructs makes up the final device which is shown below:

Genetic circuit.PNG

(AB is our antibiotic resistance cassette, ytvA is the gene controlling the light-sensing pathway, SB is the biomaterial, epsE the clutch and the 5' and 3' sections are integration sites. Light-inducible promoters are labelled with an 'L')



Modeling - Overview
Growth Curve
Genetic Circuit
Motility Analysis

Implementation

Following the design stage of our project we moved on to the implementation stage. This involved construction of a cloning strategy, construction of our biobricks and transformation and characterisation of these biobricks in B.subtilis. For more information on this aspect of the project please see the Wet Lab Hub

Implementation.PNG

Testing

The testing and validation of our project can be split into three main areas;

  • Work with B.subtilis - Including characterisation of growth curves and transformation,
  • Characterisation and control of motility
  • Production of Biomaterials in B.subtilis

Please see the Results Page for more information on the key results from the testing and validation.


Result.PNG

Achievements

Here is a summary of the achievements of the Imperial College 2008 team:

  • Submitted x..number of documented parts to the registry,
  • Characterized and improved the existing part.....,
  • Developed integration bricks, to allow devices to be constructed that can then be excised and planted into B. subtilis
  • Layed the groundwork for future teams to work with B. subtilis by BioBricking promoters, RBSs, terminators and so on and characterising them
  • Showed that expansion into other organisms is a definite possibility!
  • Helped Bristol by sending them a mini-iGEM project: Chemotactic dot-to-dot with information on quorum sensing and directed movement
  • Helped Bristol by sending them a part (BBa_J37015) from our 2007 stock which was an empty vector in the Registry


Of course, that's a very simplified description of our project. We expanded upon our project by looking into possible areas for real-world application; for a case-study of such an implementation check out how our project fits in with >>> Biocouture >>>



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