UCSF Higher-Order Systems

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<h1  align="center">CHROMATIN MEMORIES</h1>
<h1  align="center">CHROMATIN MEMORIES</h1>
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<p align="center">__________________________________________________________________________________________________</p>
 
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       <h2 align="left">Epigenetic control of gene expression</h2>
       <h2 align="left">Epigenetic control of gene expression</h2>
       <p align="left">The cells of higher eukaryotes utilize chromatin state to encode &quot;permanent&quot; epigenetic changes in gene expression.  For example, signals received by a cell during the course of development can induce the partitioning of the genome into accessible (euchromatin) and inaccessible (heterochromatin) regions that specify the fate of that cell.  This epigenetic profile, in which blocks of gene are &quot;silenced&quot; by heterochromatin, is stably maintained and inherited by daughter cells.  Thus, chromatin state provides a higher level of gene expression control that is regional (acting on many genes at once), dominant over transcription factors, ultra-cooperative (all or none), and highly stable (memory).  Engineerable control over chromatin state would clearly be a powerful tool for Synthetic Biology.  We have constructed and characterized a synthetic silencing system in <em>S. cerevisiae</em> in which we can inducibly silence specific loci in the genome. This foundational technology will facilitate the construction of complex genetic circuits with memory, and has potential application in the engineering of cell differentiation in higher eukaryotes.</p>
       <p align="left">The cells of higher eukaryotes utilize chromatin state to encode &quot;permanent&quot; epigenetic changes in gene expression.  For example, signals received by a cell during the course of development can induce the partitioning of the genome into accessible (euchromatin) and inaccessible (heterochromatin) regions that specify the fate of that cell.  This epigenetic profile, in which blocks of gene are &quot;silenced&quot; by heterochromatin, is stably maintained and inherited by daughter cells.  Thus, chromatin state provides a higher level of gene expression control that is regional (acting on many genes at once), dominant over transcription factors, ultra-cooperative (all or none), and highly stable (memory).  Engineerable control over chromatin state would clearly be a powerful tool for Synthetic Biology.  We have constructed and characterized a synthetic silencing system in <em>S. cerevisiae</em> in which we can inducibly silence specific loci in the genome. This foundational technology will facilitate the construction of complex genetic circuits with memory, and has potential application in the engineering of cell differentiation in higher eukaryotes.</p>
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           <td width="650"><h3>&nbsp;</h3>
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           <td width="650"><h3>&nbsp;</h3>
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{| style="color:#333333;background-color:#cccccc;" cellpadding="3" cellspacing="3" border="0" bordercolor="#231f26" width="99%" align="center"
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!align="center"|[[Team:UCSF|Home]]
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!align="center"|[[Team:UCSF/Team|The Team]]
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!align="center"|[[Team:UCSF/Project|The Project]]
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!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:UCSF/Modeling|Modeling]]
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!align="center"|[[Team:UCSF/Human Practices|Human Practices]]
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!align="center"|[[Team:UCSF/Notebook|Notebooks]]
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Revision as of 02:03, 24 October 2008

Untitled Document

CHROMATIN MEMORIES




Epigenetic control of gene expression

The cells of higher eukaryotes utilize chromatin state to encode "permanent" epigenetic changes in gene expression. For example, signals received by a cell during the course of development can induce the partitioning of the genome into accessible (euchromatin) and inaccessible (heterochromatin) regions that specify the fate of that cell. This epigenetic profile, in which blocks of gene are "silenced" by heterochromatin, is stably maintained and inherited by daughter cells. Thus, chromatin state provides a higher level of gene expression control that is regional (acting on many genes at once), dominant over transcription factors, ultra-cooperative (all or none), and highly stable (memory). Engineerable control over chromatin state would clearly be a powerful tool for Synthetic Biology. We have constructed and characterized a synthetic silencing system in S. cerevisiae in which we can inducibly silence specific loci in the genome. This foundational technology will facilitate the construction of complex genetic circuits with memory, and has potential application in the engineering of cell differentiation in higher eukaryotes.

More...

 

 

OUR PROJECT

Project Summary

Synthetic Chromatin Bit

    • Design
    • Properties
    • Higher-Order Systems
    • FAQs

 

 

OUR TEAM

Team members

Individual Contributions

Notebooks

Resources

FAQs

 

 

 

 

 

 


Home The Team The Project Parts Submitted to the Registry Modeling Human Practices Notebooks
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