Team:Chiba/Calendar-Home/8 September 2008

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(Difference between revisions)
(→10 September, 2008)
(→9 September, 2008)
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:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
:::->15μL?
:::->15μL?
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===9 September, 2008===
 
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-->(6/9)PCR of I9030
 
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--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
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--->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
 
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--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
 

Revision as of 07:41, 24 October 2008

>Home | Notebook

7 September 2008 <|> 9 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(7/9)

Gel Extract


--->Zymo Clean
->15μL?


Team:Output

SAP

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)①
  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤
Sample No. ①⑤
DNA(digested sample) 76
SAP/td> 22
SAP Buffer 33
TOTAL 3030
TOTAL 3030

-->37℃ 1hour

-->65℃ 30min

-->DNA purification

-->21μl 6μl

-->Gel check

Sample No. each
DNA(digested sample) 1
Loading Dye 1
dH2O 4
TOTAL 6
  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](RBS+rLuc)⑥ 33.6ng/μl
Sample No. ①&②①&③①&④①⑤&⑥⑤
DNA(vector) 333352.1
DNA(insert) 333-3.1-
Ligase 111111
Ligase Buffer(x5) 222222
dH2O 11148.94
TOTAL 101010102010
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