Team:Chiba/Calendar-Home/3 September 2008

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Revision as of 12:18, 24 October 2008

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2 September 2008 <|> 4 September 2008

Laboratory work

Team:Input

no work

Team:Communication

(2/9)--->Mini prep

[http://partsregistry.org/Part:BBa_K084009 BBa_K084009](R1, R3~R7)
[http://partsregistry.org/Part:BBa_K084010 BBa_K084010](C1,C2,C4~C8)
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154]

--->Gel Check

Sample DNA	1
Loading Dye(μL)	1
dH₂O(μL)	4
TOTAL(μL)	6

From left;

[http://partsregistry.org/Part:BBa_S03154 BBa_S03154] -> OK 33ng/μL
[http://partsregistry.org/Part:BBa_K084009 BBa_K084009](R1, R3~R7) -> OK

From left;

[http://partsregistry.org/Part:BBa_K084010 BBa_K084010](C1,C2,C4~C8) -> OK

--->Digestion test

[http://partsregistry.org/Part:BBa_K084009 BBa_K084009](R1, R3~R7)
[http://partsregistry.org/Part:BBa_K084010 BBa_K084010](C1,C2,C4~C8)

Digestion	Single	Double
Sample DNA(μL)	1	3
XbaⅠ(μL)	0.1	0.1
PstⅠ(μL)	0.1	0.1
Buffer 2(μL)	0.9	-
Buffer 3(μL)	-	-0.8
BSA(μL)	1	1
dH₂O(μL)	7	5
TOTAL(μL)	10	10

--->Gel Check

Sample DNA(μL)	10
Loading Dye(μL)	2
TOTAL(μL)	12

From left;

Single Digestion : [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]R1,R3~R7 -> OK
Single Digestion : [http://partsregistry.org/Part:BBa_K084010 BBa_K084010]C1,C2,C4~C8 -> OK

From left;

Double Digestion : [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]R1,R3~R7 -> OK
Double Digestion : [http://partsregistry.org/Part:BBa_K084010 BBa_K084010]C1,C2,C4~C8 -> OK

(2/9)--->Phenotype-test

MIX

[http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI, Competent Cells : JW1908) -> Sample Name : L1~L4
[http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI, Competent Cells : JW1908) -> Sample Name : R1~R4
[http://partsregistry.org/Part:BBa_S03623 BBa_S03623](Ptet+RBS+LuxI, Competent Cells : JW1908)
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](Ptet+RBS+LuxR+GFP, Competent Cells : JW1908)

Sample No.	1	2	3	4	5	6	7	8	9	10	11
L1	2mL	-	-	-	-	-	-	-	-	-	-
L2	-	2mL	-	-	-	-	-	-	-	-	-
L3	-	-	2mL	-	-	-	-	-	-	-	-
L4	-	-	-	2mL	-	-	-	-	-	-	-
R1	-	-	-	-	1mL	-	-	-	-	-	-
R2	-	-	-	-	-	1mL	-	-	-	-	-
R3	-	-	-	-	-	-	1mL	-	-	-	-
R4	-	-	-	-	-	-	-	1mL	-	-	-
[http://partsregistry.org/Part:BBa_S03154　BBa_S03154]	-	-	-	-	-	-	-	-	2mL	-	-
AHL(100μM)	-	-	-	-	-	-	-	-	-	-	1μL
[http://partsregistry.org/Part:BBa_T9002　BBa_T9002]	2mL	2mL	2mL	2mL	1mL	1mL	1mL	1mL	2mL	1mL	1mL
IPTG(100μM)	1μL	1μL	1μL	1μL	1μL	1μL	1μL	1μL	-	-	-

Incubated for 8hours at 37 degrees
Spindown (max rpm, 3 min)
The measurement of the intensity GFP(visual judgment)

Sample No.	1	2	3	4	5	6	7	8	9	10	11
the intensity GFP	+	+	+	+	+	+	+	++	+	+++	-

Left for 12 at room temperture.
Resuspensioned
The measurement of the intensity of GFP by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6)

Sample No.	1	2	3	4	5	6	7	8	9	10	11
the intensity GFP	25.65	18.91	20.39	21.85	26.62	26.58	30.07	33.60	27.41	10.72	55.54

Team:Output

Digestion test

[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]①
[http://partsregistry.org/Part:BBa_R0071 BBa_R0071]②
[http://partsregistry.org/Part:BBa_R0077 BBa_R0077]③
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]④
[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]⑤

Sample No.	①~⑤
DNA tamplate(μL)	5
Buffer3(μL)	1
dH₂O(μL)	4
EcoRⅠ(μL)	0.1
TOTAL(μL)	10

-->37℃30min

-->Gel check

Sample No.	①~⑤
DNA(digested sample(μL))	10
loading Dye(μL)	2
TOTAL(μL)	12

@@ Line 23: / Line 23: @@
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(μL)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>4</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>6</td>
 </tr>
@@ Line 57: / Line 57: @@
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(μL)</td>
 <td>1</td><td>3</td>
 </tr>
 <tr>
-<td>XbaⅠ</td>
+<td>XbaⅠ(μL)</td>
 <td>0.1</td><td>0.1</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstⅠ(μL)</td>
 <td>0.1</td><td>0.1</td>
 </tr>
 <tr>
-<td>Buffer 2</td>
+<td>Buffer 2(μL)</td>
 <td>0.9</td><td>-</td>
 </tr>
 <tr>
-<td>Buffer 3</td>
+<td>Buffer 3(μL)</td>
 <td>-</td><td>-0.8</td>
 </tr>
 <tr>
-<td>BSA</td>
+<td>BSA(μL)</td>
 <td>1</td><td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>7</td><td>5</td></tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
-<td>10μL</td><td>10μL</td>
+<td>10</td><td>10</td>
 </tr>
 </table>
@@ Line 96: / Line 96: @@
 :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample DNA</td>
+<td width="257">Sample DNA(μL)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(μL)</td>
 <td>2</td></tr>
 <tr><tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>12</td>
 </tr>
@@ Line 195: / Line 195: @@
 </tr>
 <tr>
-<td>DNA tamplate</td>
+<td>DNA tamplate(μL)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>Buffer3</td>
+<td>Buffer3(μL)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>4</td>
 </tr>
 <tr>
-<td>EcoRⅠ</td>
+<td>EcoRⅠ(μL)</td>
 <td>0.1</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>10</td>
 </tr>
@@ Line 226: / Line 226: @@
 </tr>
 <tr>
-<td>DNA(digestion sample)</td>
+<td>DNA(digested sample(μL))</td>
 <td>10</td>
 </tr>
 <tr>
-<td>loading Dye</td>
+<td>loading Dye(μL)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>12</td>
 </tr>
 </table>

Team:Chiba/Calendar-Home/3 September 2008

From 2008.igem.org

Revision as of 12:18, 24 October 2008

Contents

Laboratory work

Team:Input

Team:Communication

Team:Output