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Team:Warsaw/Calendar-Main/28 July 2008
From 2008.igem.org
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<h3>Cloning omega-A fusion on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> (second attempt)</h3> | <h3>Cloning omega-A fusion on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> (second attempt)</h3> | ||
<h4>Michał L., Ewa, Marcin</h4> | <h4>Michał L., Ewa, Marcin</h4> | ||
| - | <p>Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be truncated and contain only one B-domain. We inoculated bacteria containing this plasmid to obtain it for futher cloning. </p> | + | <p>Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be truncated and contain only one B-domain (which we called 'delta A'). We inoculated bacteria containing this <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2Bomega-deltaA>plasmid</a> to obtain it for futher cloning. </p> |
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<h4>Piotr</h4> | <h4>Piotr</h4> | ||
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| - | Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids pET15b+Z-alpha and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.</p></html> | + | Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BZ-alpha>pET15b+Z-alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2Bhis%2BZ%2Bomega>pET15b+Z-omega</a>. Plating them on LB with chloramphenicol and ampicillin.</p></html> |
Revision as of 17:57, 24 October 2008
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Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, MarcinSequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be truncated and contain only one B-domain (which we called 'delta A'). We inoculated bacteria containing this plasmid to obtain it for futher cloning. Protein purification: Z-alpha i Z-omegaPiotrSingle transformations of Rosetta with plasmids pET15b+Z-alpha and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.
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