Team:NTU-Singapore/Parts/Fluorescence Microscope Image

From 2008.igem.org

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=Image Results obtained from fluorescence microscope=
=Image Results obtained from fluorescence microscope=
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Below are Images obtained from the microscope on the various standards that iGEM MIT has provided for us this year.  
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Below are Images obtained from the microscope of the various standards (Low, Medium, High & Standard) that iGEM Headquarters has provided for us in the [http://partsregistry.org/Measurement/SPU/Learn iGEM Newsletter].  
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==Standard 102==
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==Weak promoter [http://partsregistry.org/Part:BBa_J23150 BBa_J23150]==
{| align="center" border="1"
{| align="center" border="1"
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|[[Image:102-norm_030708.JPG|thumb|400px|102 Normal]]
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|[[Image:102-norm_030708.JPG|thumb|400px|BBa_J23150 Before Excitation]]
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|[[Image:102_gfp_030708.jpg|thumb|400px|102 GFP]]
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|[[Image:102_gfp_030708.jpg|thumb|400px|BBa_J23150 After Excitation]]
|}
|}
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==Standard 150==
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==Medium promoter [http://partsregistry.org/Part:BBa_J23151 BBa_J23151]==
{| align="center" border="1"
{| align="center" border="1"
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|[[Image: 150_norm_030708.JPG|thumb|400px|150 Normal]]
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|[[Image: 150_norm_030708.JPG|thumb|400px|BBa_J23151 Before Excitation ]]
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|[[Image: 150_gfp_030708.JPG|thumb|400px|150 GFP]]
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|[[Image: 150_gfp_030708.JPG|thumb|400px|BBa_J23151 After Excitation ]]
|}
|}
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==Standard 151==
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==Strong promoter [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] ==
{| align="center" border="1"
{| align="center" border="1"
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|[[Image: 151_norm_030708.JPG|thumb|400px|151 Normal]]
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|[[Image: 151_norm_030708.JPG|thumb|400px|BBa_J23102 Before Excitation]]
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|[[Image: 151_gfp_030708.JPG|thumb|400px|151 GFP]]
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|[[Image: 151_gfp_030708.JPG|thumb|400px|BBa_J23102 After Excitation]]
|}
|}
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==Standard 260==
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==Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]  ==
{| align="center" border="1"
{| align="center" border="1"
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|[[Image:260norm_030708.JPG|thumb|400px|260 Normal]]
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|[[Image:260norm_030708.JPG|thumb|400px|BBa_I20260Before Excita]]
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|[[Image:260gfp_030708_b.JPG|thumb|400px|260 GFP]]
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|[[Image:260gfp_030708_b.JPG|thumb|400px|BBa_I20260After Excitation]]
|}
|}
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The above picture shows the green fluorescence produced by Top 10 ecoli cells under blue light excitation. These Ecoli cells contain the standard promoter 260 with GFP and thye have been allowed to grow to an OD of 1.2 before a snap shot is taken.
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The above pictures showed the green fluorescence protein produced by Top 10 ecoli cells taken under blue light excitation. These Ecoli cells contain the standard promoters with GFP and they have been allowed to grow to an OD of 1.2 before a snap shot was taken.
=Video obtained from fluorescence microscope=
=Video obtained from fluorescence microscope=

Revision as of 13:51, 24 October 2008

Contents

pLacI-GFP part:BBa_J04430 found to express RFP instead

The below pictures were taken after the biobrick part: BBa_J04430 was transformed into Top 10 competent cells. They clearly showed, either under normal light or UV light, the cells appears red or glow red respectively. This is contrary to what was expected, as we would expect it to glow green under UV light. This problem was highlighted to the iGEM Headquarters. We decided to ligate the pLac promoter (R0010) with the GFP (E0040) ourselves and the result was good this time round as the cells glow green under UV light as expected.

Cells appears pinkish even under normal light
Cells glow PINK under UV light

Image Results obtained from fluorescence microscope

Below are Images obtained from the microscope of the various standards (Low, Medium, High & Standard) that iGEM Headquarters has provided for us in the [http://partsregistry.org/Measurement/SPU/Learn iGEM Newsletter].

Weak promoter [http://partsregistry.org/Part:BBa_J23150 BBa_J23150]

BBa_J23150 Before Excitation
BBa_J23150 After Excitation

Medium promoter [http://partsregistry.org/Part:BBa_J23151 BBa_J23151]

BBa_J23151 Before Excitation
BBa_J23151 After Excitation

Strong promoter [http://partsregistry.org/Part:BBa_J23102 BBa_J23102]

BBa_J23102 Before Excitation
BBa_J23102 After Excitation

Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]

BBa_I20260Before Excita
BBa_I20260After Excitation

The above pictures showed the green fluorescence protein produced by Top 10 ecoli cells taken under blue light excitation. These Ecoli cells contain the standard promoters with GFP and they have been allowed to grow to an OD of 1.2 before a snap shot was taken.

Video obtained from fluorescence microscope


Video of E coli under normal white light



Video of E coli under blue light excitation
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