Team:Warsaw/Calendar-Main/30 September 2008

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<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of isolated plasmids with EcoRI and BcuI (BamHI buffer). </li>
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of isolated plasmids with EcoRI and BcuI (BamHI buffer). </li>
<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pACYC177+OmpA_omega_deltaA_alpha with BamHI and BglII (BamHI buffer). </li>
<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pACYC177+OmpA_omega_deltaA_alpha with BamHI and BglII (BamHI buffer). </li>
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<li>Gel elctrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band: ??????. </li> </li>
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<li>Gel elctrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band: ??????. </li> </ol>
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<h4>Piotr</h4>
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<ol><li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#electrotransform">electroporation</a> with overnight ligations (RFP(??????) + Z and RFP(????) + OmpA_link). </li>
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<li>Plating on LB + ampicillin.</li>
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</ol>
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<li>Gel elctrophoresis. Chioce of proper clone. </li>
 

Revision as of 14:03, 24 October 2008

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Mutagenesis of protein A

Paweł

Results of sequencing: unfortunately all sequences where wild-type.

Preparation of BioBricks

Michał K.

  1. Isolation of HQs(??????) plasmids.
  2. Digest of isolated plasmids with EcoRI and BcuI (BamHI buffer).
  3. Digest of pACYC177+OmpA_omega_deltaA_alpha with BamHI and BglII (BamHI buffer).
  4. Gel elctrophoresis and gel-out of proper band: ??????.

Piotr

  1. E. coli TOP10 electroporation with overnight ligations (RFP(??????) + Z and RFP(????) + OmpA_link).
  2. Plating on LB + ampicillin.

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