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Team:NTU-Singapore/Parts
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==Introduction== | ==Introduction== | ||
The group proposed to discuss and investigate the working range of the parts and devices of our system | The group proposed to discuss and investigate the working range of the parts and devices of our system | ||
| - | *The proposed variables to investigate are: <br> i) Concentration of activator<br> | + | *The proposed variables to investigate are: <br> |
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| + | i) Concentration of activator<br> | ||
ii) Time <br> | ii) Time <br> | ||
Revision as of 14:47, 24 October 2008
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Introduction
The group proposed to discuss and investigate the working range of the parts and devices of our system
- The proposed variables to investigate are:
i) Concentration of activator
ii) Time
iii) Temperature
Characterization of Parts
We are interested in characterizing the following parts:
i) Lactose/IPTG sensitive Promoter pLac [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]
ii) Iron Sensitive Promoter pFe [http://partsregistry.org/wiki/index.php?title=Part:BBa_I716014 BBa_I716014]
iii) pLsrA-YFP
The characterization is carried out by ligating the desired promoter with the Green Fluorescence protein: [http://partsregistry.org/Part:BBa_E0240 BBa_E02040]
- For the pLac BBa_R0010, it is already available in the form that we desired in the registry as [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]
The FLx800™ Fluorescence Microplate Reader
The FLx800™ Fluorescence Microplate Reader was available for our use courtesy of Division of Bio-engineering lab. It was purchased from BioTek and for more information about the reader, you can refer to [http://www.biotek.com/products/product_detail.php?pid=117 The FLx800™ Fluorescence Microplate Reader]
The FLx800™ Fluorescence Microplate Reader has a computer software KCJunior that was installed in a computer adjacent to the reader for collection and processing of data from our samples.
