Team:Warsaw/Calendar-Main/11 September 2008

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<p>Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicylin with minimum concentration of prey (yesterday results).<br><br><br><br>
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<p><ol><li>Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicylin with minimum concentration of prey (yesterday results).</li>
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<li>Mesurment of growth after 6 hours - 2x większy wzrost bakterii z białkiemo ddziałującym (Omp_A_omega 2x więcej niż Omp_omega z białkiem Z_alpha itd...)!!!
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<br><br><br><br>
We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</p><br><br>
We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</p><br><br>

Revision as of 15:16, 24 October 2008

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Tests for ampicillin resistance given by protein added to medium

Piotr

  1. Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicylin with minimum concentration of prey (yesterday results).
  2. Mesurment of growth after 6 hours - 2x większy wzrost bakterii z białkiemo ddziałującym (Omp_A_omega 2x więcej niż Omp_omega z białkiem Z_alpha itd...)!!!



    We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!



    Repetition of test for ampicillin resistance given by protein added to medium

    Michał L., Ewa, Marcin

    1. We follow the protocol.
      • Inoculation of E. coli TOP10 carrying OmpA variants into liquid LB with kanamycin and IPTG.

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