Wiki/Team:Warsaw/igem notebook.htm
From 2008.igem.org
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o">Purification of His_Z_alpha and His_Z_omega</a></li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o">Purification of His_Z_alpha and His_Z_omega</a></li> | ||
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<h4>If not stated otherwise, all agarose gels bear <a href="http://www.fermentas.com/catalog/electrophoresis/generulers.htm#Mix">Fermentas GeneRuler (100-10000 bp)</a> ladder. PAGE gels bear <a href="http://www.fermentas.com/catalog/electrophoresis/marksm0671.htm">PageRuler Fermentas Prestained protein ladder</a>.</h4> | <h4>If not stated otherwise, all agarose gels bear <a href="http://www.fermentas.com/catalog/electrophoresis/generulers.htm#Mix">Fermentas GeneRuler (100-10000 bp)</a> ladder. PAGE gels bear <a href="http://www.fermentas.com/catalog/electrophoresis/marksm0671.htm">PageRuler Fermentas Prestained protein ladder</a>.</h4> | ||
- | <h2 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers"> | + | <h2 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a></h2> |
- | <h2 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors"> | + | <h2 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a></h2> |
- | <h2 align="left"> | + | <h2 align="left">Strains</h2> |
<h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4> | <h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4> | ||
<p>F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)</p> | <p>F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)</p> |
Revision as of 16:26, 24 October 2008
Protocols
If not stated otherwise, all agarose gels bear Fermentas GeneRuler (100-10000 bp) ladder. PAGE gels bear PageRuler Fermentas Prestained protein ladder.PrimersVectorsStrainsRosetta(DE3)pLysSF- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR) TOP10 (Invitrogen)F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- NR9458 (IBB collection; in notebook called mutD5)ara thi Δ pro lac mutD5 zaf-13 ::Tn10 GM2163ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |