Team:Tokyo Tech/Result

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== <font size=5> Checking pressure response ability of Plac </font> ==
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<p class=MsoNormal>We examined changing of GFP fluorescence strength with pressurizing.At the same time, we examined fluorescence strength by adding IPTG under 0.1MPa.As a way of observation, we adopted a fluorescence microscope, FACS and FLA.FACS and FLA is for quantitative analysis of fluorescence strength. FLA’s result is Fig2, Fig3, Fig4.</font></p>
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<p class=MsoNormal>Fig 2: Average of Plac /p></td>
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<td class="a" width="40%">　Fig 3: Average of ⊿p </td>
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<p class=MsoNormal>Fig 2: Average of Plac /p></td>
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<td class="a" width="40%">　Fig 3: Average of ⊿p </td>
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          <td class="a">　Fig 4: Average of Ptet</td>
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          <td class="a" width="40%"> Fig 5: Fig2&3&4 gathers into one chart.</td>
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<p class=MsoNormal>Figure 2, 3, 4 ; These charts show change of Geo Mean under each pressures. Figure 2 is about Plac-GFP. We constructed Plac-GFP. E. coli strains is JM109. Between 0.1MPa and 30MPa, Geo Mean almost don’t change.But at 50MPa Geo Mean increase sharply. Figure 3 is about promoter less-GFP (∆p-GFP) as a negative control. Between 0.1MPa and 30MPa, Geo Mean almost don’t change. But, at 50MPa Geo Mean increase sharply. Figure 4, is about Ptet-GFP as a positive control.Increasing pressure, Geo Mean increases. </p></td>
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A fluorescence microscope is for qualitative analysis of fluorescence strength. Fluorescence microscope image is Fig 6.</td>
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Revision as of 18:21, 24 October 2008

Home Construction Acrylic container Development of promoter Genetic toggle switch Parts Submitted to the Registry Result The Team Notebook


Checking pressure response ability of Plac

　

We examined changing of GFP fluorescence strength with pressurizing.At the same time, we examined fluorescence strength by adding IPTG under 0.1MPa.As a way of observation, we adopted a fluorescence microscope, FACS and FLA.FACS and FLA is for quantitative analysis of fluorescence strength. FLA’s result is Fig2, Fig3, Fig4.

　


　

Fig 2: Average of Plac /p>

 　Fig 3: Average of ⊿p
　

Fig 2: Average of Plac /p>

 　Fig 3: Average of ⊿p
　 　Fig 4: Average of Ptet Fig 5: Fig2&3&4 gathers into one chart.
　

Figure 2, 3, 4 ; These charts show change of Geo Mean under each pressures. Figure 2 is about Plac-GFP. We constructed Plac-GFP. E. coli strains is JM109. Between 0.1MPa and 30MPa, Geo Mean almost don’t change.But at 50MPa Geo Mean increase sharply. Figure 3 is about promoter less-GFP (∆p-GFP) as a negative control. Between 0.1MPa and 30MPa, Geo Mean almost don’t change. But, at 50MPa Geo Mean increase sharply. Figure 4, is about Ptet-GFP as a positive control.Increasing pressure, Geo Mean increases.

　
　 A fluorescence microscope is for qualitative analysis of fluorescence strength. Fluorescence microscope image is Fig 6.

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