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Team:Chiba/Calendar-Home/23 August 2008
From 2008.igem.org
(Difference between revisions)
(→Laboratory work) |
(→Team:Communication) |
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| Line 44: | Line 44: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>EcoRI</td> |
<td>-</td><td>-</td><td>0.1</td><td>0.1</td> | <td>-</td><td>-</td><td>0.1</td><td>0.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>PstI</td> |
<td>-</td><td>-</td><td>0.1</td><td>0.1</td> | <td>-</td><td>-</td><td>0.1</td><td>0.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Buffer | + | <td>Buffer EcoRI</td> |
<td>-</td><td>-</td><td>0.9</td><td>0.8</td> | <td>-</td><td>-</td><td>0.9</td><td>0.8</td> | ||
</tr> | </tr> | ||
| Line 65: | Line 65: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>3μl</td><td>1μl</td><td>10μl</td><td>10μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 94: | Line 94: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>6μl</td><td>6μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 129: | Line 129: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>12μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 169: | Line 169: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>EcoRI</td> |
<td>-</td><td>0.1</td><td>0.1</td> | <td>-</td><td>0.1</td><td>0.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>SpeI</td> |
<td>-</td><td>-</td><td>0.1</td> | <td>-</td><td>-</td><td>0.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>EcoRI Buffer</td> |
<td>-</td><td>0.9</td><td>0.8</td> | <td>-</td><td>0.9</td><td>0.8</td> | ||
</tr> | </tr> | ||
| Line 185: | Line 185: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>dH<sub>2</sub>O</td> |
<td>-</td><td>5</td><td>5</td> | <td>-</td><td>5</td><td>5</td> | ||
</tr> | </tr> | ||
| Line 213: | Line 213: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>dH<sub>2</sub>O</td> |
<td>2</td><td>-</td><td>-</td> | <td>2</td><td>-</td><td>-</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>6μl</td><td>12μl</td><td>12μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 19:57, 29 October 2008
22 August 2008 <|> 24 August 2008
Contents |
Meeting with UmeG
the ppt for our weekly meeting(japanese)
- Team:Input
- Team:Communication
- team:Output
Laboratory work
Team:Input
no work
Team:Communication
- (21/8)--->Mini prep
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
- --->Digestion test
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
Sample No. 1- 6 7 1 - 7 1 - 7 DNA 3 1 3 3 EcoRI - - 0.1 0.1 PstI - - 0.1 0.1 Buffer EcoRI - - 0.9 0.8 BSA - - 1 1 dH2O - - 5 5 TOTAL 3μl 1μl 10μl 10μl
- --->Gel Check
Sample No. 1~6 7 Sample DNA 3 1 Loading Dye 1 1 dH2O 2 4 TOTAL 6μl 6μl - From left; Sanple No. 1~7
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
- From left; Sanple No. 1~7
Sample No. 8~21 Sample DNA 10 Loading Dye 2 TOTAL 12μl - From left;
- (Single Digestion) Sanple No. 8~14
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
- (Double Digestion) Sample No. 15~21
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK?
- Digestion test
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007:old-sample)
Sample No. 1 2 3 Sample DNA 3 3 3 EcoRI - 0.1 0.1 SpeI - - 0.1 EcoRI Buffer - 0.9 0.8 BSA - 1 1 dH2O - 5 5 TOTAL 3 10 10
--->Gel Check
Sample No. 1 2 3 Sample DNA 3 10 10 Loading Dye 1 2 2 dH2O 2 - - TOTAL 6μl 12μl 12μl - From left;
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Single Digestion -> OK
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Double Digestion -> Bad
- From left;
Team:Output
no work
