Team:Chiba/Calendar-Home/16 September 2008

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===Team:Communication===
===Team:Communication===
(15/9)->
(15/9)->
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*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
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[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
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**conditions
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*[[Team:Chiba/protocol/transformation|Transformed]] these plasmids　into E.coli strain JW1908.(also transformed [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] into E.Coli strain JW1908.)
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#strain<br>Senders:JW1908<br>Receiver:JW1908<br>
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**[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
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#temparature<br>37℃ and 30℃
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**[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
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**[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
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**[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
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*conditions
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#temparature<br>37℃ and 30℃
===Team:Output===
===Team:Output===

Revision as of 12:12, 26 October 2008

>Home | Notebook

15 September 2008 <|> 17 September 2008

Laboratory work

Team:Communication

(15/9)-> time-delay test

  • Transformed these plasmids　into E.coli strain JW1908.(also transformed [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] into E.Coli strain JW1908.)
    • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
    • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
    • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
    • [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]


  • conditions
  1. temparature
    37℃ and 30℃

Team:Output


Digestion test

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(x2)
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(x5)
  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(x2)
  • [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
  • [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
  • [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](RBS+cinR)
Sample No. each
DNA(μL) 3
EcoRⅠ(μL) 0.083
PstⅠ(μL) 0.083
BSA(x10)(μL) 1
Buffer3(x10)(μL) 1
dH2O(μL) 5
TOTAL(μL) 10

-->37℃ 1hour

Gel check

Sample No. each
DNA(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12

Transformation

  • [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](LuxI+RBS+LuxR)->no colony
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](LuxI+LuxR+pLux+GFP)
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

PCR

  • Venus YFP(ptet+LuxR+YFP)
Sample No. YFP
DNA(μL) 1
Venus Fwd(μL) 5
Rev(VR)(μL) 5
Thermo pol Buffer(μL) 10
dNTP mix(μL) 10
Vent(μL) 1
dH2O(μL) 68
TOTAL(μL) 100
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