Team:Chiba/Calendar-Home/17 September 2008

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*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
**conditions
**conditions
-
#strain<br>Senders:JW1908<br>Receiver:JW1908<br>
+
#E.coli strain<br>Senders:JW1908<br>Receiver:JW1908<br>
-
#temparature<br>37℃ and 30℃
+
#reaction temparature<br>37℃ and 30℃
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
**conditions
**conditions
-
#strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
+
#E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
-
#temparature<br>37℃ and 30℃
+
#reaction temparature<br>37℃ and 30℃
-
 
+
===Team:Output===
===Team:Output===

Revision as of 12:36, 26 October 2008

>Home | Notebook

16 September 2008 <|> 18 September 2008

Laboratory work

Team:Communication

  1. E.coli strain
    Senders:JW1908
    Receiver:JW1908
  2. reaction temparature
    37℃ and 30℃
  1. E.coli strain
    Senders:XL10GOLD
    Receiver:JW1908
  2. reaction temparature
    37℃ and 30℃

Team:Output

Transformation

  • [http://partsregistry.org/Part:BBa_I13500 BBa_I13500](RBS+GFP)
  • [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](Luxpr+LuxR)-->no colony

miniprepped

  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](Luxpr+LuxR+LuxR+GFP)
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

Digestion

  • [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](ptetR+RBS)
  • [http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
  • Ligation product(ptet+LasR)
  • Ligation product(ptet+RhlR)
  • Ligation product(ptet+cinR)
Sample No. each
DNA template(μL) 25
EcoRⅠ(μL) 1
PstⅠ(μL) 1
BSA(x10)(μL) 4
EcoRⅠ Buffer(μL) 4
dH2O(μL) 5
TOTAL(μL) 40

-->Left at37 degrees for 1 hour.

-->Next day

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