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Team:Chiba/Receiver experiments
From 2008.igem.org
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===LuxR/Plux mutants=== | ===LuxR/Plux mutants=== | ||
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===AiiA Receiver=== | ===AiiA Receiver=== | ||
[[Image:AiiA-Receiver Chiba.gif|frame|right|'''Fig.6''' AiiA Receiver]] | [[Image:AiiA-Receiver Chiba.gif|frame|right|'''Fig.6''' AiiA Receiver]] | ||
Revision as of 05:03, 25 October 2008
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Project Design ( Sender 実験 | レシーバー実験 ) Making time delay | Our Goal |
Contents |
LuxR/Plux mutants
method
result
AiiA Receiver
Fig.6 AiiA Receiver
Design
オートインデューサー不活性化酵素であるAiiAをレシーバー菌で発現させることにより、レシーバー菌内でのAHL濃度の増加が遅くなるため、AiiAを発現しないレシーバー菌に比べて、GFP発現が遅くなる。
Experiment
- Sender
- [http://partsregistry.org/Part:BBa_S03623 BBa_S03623 (AHL auto inucer)]
method
result
- Receivers
- AiiA Receiver
- non-AiiA Receiver
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