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Team:Chiba/Sender experiments
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===Quorum-Sensing Cross-talk=== | ===Quorum-Sensing Cross-talk=== | ||
| - | + | [[Image:Sender crosstalk chiba.gif|frame|right|'''Fig.5''' Senders crosstalk]] | |
*'''Senders''' | *'''Senders''' | ||
**[http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI] | **[http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI] | ||
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**[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)] | **[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)] | ||
[[Image:Reporter 9002 chiba.gif]] | [[Image:Reporter 9002 chiba.gif]] | ||
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===Method=== | ===Method=== | ||
Revision as of 05:16, 25 October 2008
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Project Design ( Sender experiments | Receiver experiments ) | Our Goal |
Quorum-Sensing Cross-talk
Fig.5 Senders crosstalk
- Senders
- [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]
- [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]
- [http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]
- Receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
Method
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
- Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 37°C or 30°C.
- Measured intensity of green fluorescence at regular time intervals.
Result
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