"
Team:Chiba/Sender experiments
From 2008.igem.org
(Difference between revisions)
(→Quorum-Sensing Cross-talk) |
(→Quorum-Sensing Cross-talk) |
||
| Line 15: | Line 15: | ||
| - | + | ==Quorum-Sensing Cross-talk== | |
[[Image:Sender crosstalk chiba.gif|frame|right|'''Fig.5''' Senders crosstalk]] | [[Image:Sender crosstalk chiba.gif|frame|right|'''Fig.5''' Senders crosstalk]] | ||
*'''Senders''' | *'''Senders''' | ||
Revision as of 06:57, 25 October 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Notebook |
|---|
|
Project Design ( Sender experiments | Receiver experiments ) | Our Goal |
Quorum-Sensing Cross-talk
Fig.5 Senders crosstalk
- Senders
- [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]
- [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]
- [http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]
- Receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
Method
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
- Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 37°C or 30°C.
- Measured intensity of green fluorescence at regular time intervals.
Result
| Home | The Team | The Project | Parts Submitted to the Registry | Notebook |
|---|
