Team:Chiba/Sender experiments

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Revision as of 10:43, 25 October 2008

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Project Design ( Sender experiments | Receiver experiments ) | Our Goal

Quorum-Sensing Cross-talk

Purpose

Fig.5 Senders crosstalk

Senders

[http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]

[http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]

[http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]

Receiver

[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

Method

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
Inoculated them independently in liquid media. Incubated at 37°C 12h
Mixed them.
Incubated at 30°C.
Measured intensity of green fluorescence at regular time intervals.

Result

1.Crosstalk test at 30°C

Fig. senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.Labelling LuxI,RhlI,LasI means Fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.

LasI
RhlI

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@@ Line 43: / Line 43: @@
 ====1.Crosstalk test at 30°C ====
 [[image:senders_crosstalk_chiba_01.gif|frame|left|
-'''Fig.''' senders crosstalk test.
+'''Fig.''' senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.Labelling LuxI,RhlI,LasI means Fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.]]<br clear="all">
-senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.
-]]<br clear="all">
 *LasI