Team:NTU-Singapore/Notebook/25 July 2008

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Latest revision as of 02:33, 28 October 2008

Friday 25 July

  • Darius
    • Miniprep 23 tubes of bacteria, E7-ter, Lysis Terminator, lysis, lsrA respectively.
  • Digestion of E7-terminator, lysis Terminator, Lysis Standard Vector, LsrA standard vector.

Heat inactivation at 80 celcius in thermal cycler before gel run.

  • Run Gel Check
    • E7 showed a 2k Band, however, this is not conclusive as GFP vector and E7 Is of the same size.

Lysis standard vector is successful, showed a band between 100bp and 200bp(140bp) LsrA standard vector failed. Lysis Terminator was kept in the fridge for saturday gel run due to time constraits i had on friday.

  • PCR verification of E7 terminator. Ran PCR with different constituents.
    • Old E7 Primers, New E7 primers, E7 verification primers.
  • Results seen on saturday morning.
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