Team:Warsaw/Calendar-Main/19 June 2008

From 2008.igem.org

(Difference between revisions)
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<h3> Blue/white test </h3>
<h3> Blue/white test </h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
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<ol>
<li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br>
<li>The analysis of lacZ sequence from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>:<br>
There is no mutation in beta-galactosidase gene. Why are the colonies white?</li>
There is no mutation in beta-galactosidase gene. Why are the colonies white?</li>
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Template: DNA isolated from white colonies<br>
Template: DNA isolated from white colonies<br>
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Primers: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqR">pZCseqR</a><br>
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Primers: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqR">pZCseqR</a></ol><br>
<br>
<br>
<table id="result">
<table id="result">

Revision as of 16:33, 25 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

Blue/white test

Piotr

  1. The analysis of lacZ sequence from pZC320:
    There is no mutation in beta-galactosidase gene. Why are the colonies white?
  2. Colony PCR.
    Template: DNA isolated from white colonies
    Primers: pZCseqL and pZCseqR


Colony PCR program
TemperatureTimeNo. of cycles
94°C4:00
94°C0:3028 cycles
48°C0:45
72°C1:30
72°C10:00
4°Cinfinite

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