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Team:Chiba/Sender experiments
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====1.Crosstalk test==== | ====1.Crosstalk test==== | ||
[[image:senders_crosstalk_chiba_01.gif|frame|left| | [[image:senders_crosstalk_chiba_01.gif|frame|left| | ||
| - | '''Fig.''' senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.]]<br clear="all"> | + | '''Fig.''' senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.]]<br clear="all"> |
| - | *RhlIとLuxIでは、GFP | + | *RhlIとLuxIでは、GFP inductionにかかる時間はほとんど同じであった. |
| - | *LasIは、GFP | + | |
| + | *LasIは、GFP inductionが他より約2時間遅れた. | ||
| + | |||
| + | |||
| + | （冨永） | ||
====2.Demonstration==== | ====2.Demonstration==== | ||
Revision as of 00:16, 26 October 2008
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Contents |
Quorum-Sensing Cross-talk
Purpose
To Confirm that communication using non-endogenous molecules results in slower activation of receivers.
冨永
Fig.5 Senders crosstalk
Senders
- [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]
- [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]
- [http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]
Receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
Method
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
- Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 30°C.
- Measured intensity of green fluorescence at regular time intervals.
Result
1.Crosstalk test
Fig. senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.
- RhlIとLuxIでは、GFP inductionにかかる時間はほとんど同じであった.
- LasIは、GFP inductionが他より約2時間遅れた.
（冨永）
2.Demonstration
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