Team:Warsaw/Calendar-Main/10 October 2008

From 2008.igem.org

(Difference between revisions)
Line 17: Line 17:
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested  5kb (??????) vector from (30 September) with pT7_omega_link and  RFP(?????) with pT7_alpha fragment (1 hr).</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested  5kb (??????) vector from (30 September) with pT7_omega_link and  RFP(?????) with pT7_alpha fragment (1 hr).</li>
 +
 +
</ol>
<h3></h3>
<h3></h3>
<h4>Piotr</h4>
<h4>Piotr</h4>

Revision as of 14:45, 25 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Visit in US Embassy

Visas have been accorded to the whole team.

Preparation of BioBricks

Michał K.

  1. Digest of RFP(?????)+deltaA with EcoRI and SacI (BamHI buffer), dephosphorylation with CIAP
  2. Gel electrophoresis and gel-out of proper band 2200 bp.
  3. Ligation of digested 5kb (??????) vector from (30 September) with pT7_omega_link and RFP(?????) with pT7_alpha fragment (1 hr).

Piotr

    Piotr

    1. Transformation of TOP10 with above ligations.
    2. Tranformants planting on LB with kanamycin.


Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/10_October_2008"