Team:Warsaw/Calendar-Main/9 September 2008

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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4>
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<p>Inoculation of TOP10 carrying OmpA_A_alpha, OmpA_A_omega and OmpA_Z_omega to LB with inducer (0.25 mM IPTG) and kanamycin.</p>
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<p>Inoculation of TOP10 carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> to LB with inducer (0.25 mM IPTG) and kanamycin.</p>

Revision as of 20:23, 25 October 2008

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Purification of proteins: A-alpha

Piotr

Measurement of concentration of protein(BCA method).

Tests for ampicillin resistance given by protein added to medium

Piotr

Inoculation of TOP10 carrying pACYC177+OmpA_A_alpha, pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega to LB with inducer (0.25 mM IPTG) and kanamycin.

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