Team:Warsaw/Calendar-Main/19 September 2008

From 2008.igem.org

(Difference between revisions)
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<h3>MutD<sub>5</sub> testing</h3>
<h3>MutD<sub>5</sub> testing</h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
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Testing MutD<sub>5</sub> ampicillin resistance - MutD<sub>5</sub> has not spontaneous ampicillin resistance mutations.
+
<p>Testing MutD<sub>5</sub> ampicillin resistance - MutD<sub>5</sub> has not spontaneous ampicillin resistance mutations.</p>
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<p>
<p>
Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)             
Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)             
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<ol><li><ul><li>Omp_A_omega, Omp_omega and Omp_Z_omega - A_alpha protein added ~100 μg </li>  
+
<ol><li><ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - A_alpha protein added ~100 μg </li>  
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<li>Omp_A_omega, Omp_omega and Omp_Z_omega - Z_alfa protein added ~100 μg</li>  
+
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - Z_alpha protein added ~100 μg</li>  
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<li>Omp_A_alpha, Omp_alpha and Omp_Z_alpha - Z_omega protein added ~100 μg</li> </ul></p>
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<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> - Z_omega protein added ~100 μg</li> </ul></p>
</li><li>Bacteria centrifuged and frozen
</li><li>Bacteria centrifuged and frozen
</li></ol>
</li></ol>
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<ol>
<ol>
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments of RFP(??????) and deltaA. </li></ol>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> and &Delta;A. </li></ol>
<h3></h3>
<h3></h3>

Revision as of 23:08, 25 October 2008

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MutD5 testing

Piotr

Testing MutD5 ampicillin resistance - MutD5 has not spontaneous ampicillin resistance mutations.

'Hunter and prey' system tests: Competition tests

Weronika

Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)

  2. Bacteria centrifuged and frozen

Preparation of BioBricks

Michał K.

  1. Gel electrophoresis to estimate concentration of purified DNA from previous day.
  2. Ligation of isolated DNA fragments of pSB1A3 and ΔA.

Piotr

  1. E. coli TOP10 transformation with the above ligation.
  2. Plating on LB + ampicillin.

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