Team:Warsaw/Calendar-Main/22 October 2008

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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day pACYC177 + OmpA_alpha_ and RFP(?????) + AraC+pBAD+AID.</li>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found only for pACYC177 + OmpA_alpha_.</li></ol>
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<h4>Piotr</h4>
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<li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day pACYC177 + OmpA_omega_.</li>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.</li>
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Revision as of 20:37, 25 October 2008

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Preparation of BioBricks

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA_alpha_ and RFP(?????) + AraC+pBAD+AID.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found only for pACYC177 + OmpA_alpha_.

Piotr

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA_omega_.
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

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