Team:Warsaw/Calendar-Main/20 October 2008

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<h3>Preparation of pT7_alpha_link</h3>
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<h4>Michał K.</h4>
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<p>Inoculation of colonies from plate with ligation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB2K3>pSB2K3</a> (from 30 September) + pT7_alpha to LB with kanamycin.</p>
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<h3>Preparation of BioBricks</h3>
<h3>Preparation of BioBricks</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>

Revision as of 09:20, 27 October 2008

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Preparation of pT7_alpha_link

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 (from 30 September) + pT7_alpha to LB with kanamycin.

Preparation of BioBricks

Michał K.

  1. Inoculation of colonies from plate with ligation of 5kb(?????) + pT7_alpha to LB with kanamycin and RFP(?????) + AraC+pBAD+AID to LB with ampicillin.
  2. Digest of 3kb(????)+pLac_OmpA_alpha and RFP(????)+OmpA with EcoRI and SacI (BamHI buffer).
  3. Gel electrophoresis and gel-out of proper band 500 bp for RFP(????)+OmpA and 3000 bp for 3kb(????)+pLac_OmpA_alpha.
  4. Ligation of DNA fragments: 3kb(?????)_ alpha + Omp.

Piotr

  1. Transformation of TOP10 with above ligation.
  2. Tranformants planting on LB with kanamycin.

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