Team:Warsaw/Calendar-Main/17 September 2008
From 2008.igem.org
(Difference between revisions)
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<p><ol><li>Plasmid isolation from previous day's cultures.</li><li>Digest with SacI and BamHI.</li><li>Electrophoresis.</li></ol></p> | <p><ol><li>Plasmid isolation from previous day's cultures.</li><li>Digest with SacI and BamHI.</li><li>Electrophoresis.</li></ol></p> | ||
- | [photo of | + | [photo of gel goes here!] |
+ | |||
<p>Conclusion: cells with interracting protein survive competition!</p> | <p>Conclusion: cells with interracting protein survive competition!</p> | ||
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primers (elongation length 90s) to obtain OmpA_omega fragment. </li> | primers (elongation length 90s) to obtain OmpA_omega fragment. </li> | ||
<p>Each reaction was carried out with 25 cycles and in temperature gradient from 55 to 75 °C (four reactions).</p> | <p>Each reaction was carried out with 25 cycles and in temperature gradient from 55 to 75 °C (four reactions).</p> | ||
- | <li> Gel electrophoresis of PCR products.</li> | + | <li> Gel electrophoresis of PCR products <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/17_September_2008#fig1">Fig. 1</a>.</li> |
</ol> | </ol> | ||
+ | |||
+ | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/9/9e/Grad_17_09.jpg" width=300/></a><var><b>Fig. 1. Gradient PCR (temperatures:55-75 °C)</b><br> | ||
+ | 1. Marker<br> | ||
+ | 2. 60 °C deltaA<br> | ||
+ | 3. 65 °C deltaA<br> | ||
+ | 4. 70 °C deltaA<br> | ||
+ | 5. 75 °C deltaA<br> | ||
+ | 6. 60 °C omega<br> | ||
+ | 7. 65 °C omega<br> | ||
+ | 8. 70 °C omega<br> | ||
+ | 9. 75 °C omega<br> | ||
+ | 10. 60 °C alpha<br> | ||
+ | 11. 65 °C alpha<br> | ||
+ | 12. 70 °C alpha<br> | ||
+ | 13. 60 °C alpha<br> | ||
+ | 14. 65 °C OmpA_omega<br> | ||
+ | 15. 70 °C OmpA_omega<br> | ||
+ | 16. 75 °C OmpA_omega<br> | ||
+ | 17. 75 °C OmpA_omega<br></var> | ||
+ | |||
+ | |||
+ | |||
</html> | </html> | ||
Revision as of 22:01, 26 October 2008
'Hunter and prey' system tests: Competition testsPiotr
Conclusion: cells with interracting protein survive competition! MutD5 testingEmiliaInoculation of MutD5 into medium with tetracycline. Optimisation of primers for preparation of partsMichał K.
Each reaction was carried out with 25 cycles and in temperature gradient from 55 to 75 °C (four reactions). 1. Marker 2. 60 °C deltaA 3. 65 °C deltaA 4. 70 °C deltaA 5. 75 °C deltaA 6. 60 °C omega 7. 65 °C omega 8. 70 °C omega 9. 75 °C omega 10. 60 °C alpha 11. 65 °C alpha 12. 70 °C alpha 13. 60 °C alpha 14. 65 °C OmpA_omega 15. 70 °C OmpA_omega 16. 75 °C OmpA_omega 17. 75 °C OmpA_omega
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