Team:Warsaw/Calendar-Main/19 September 2008

From 2008.igem.org

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<h3>Preparation of BioBricks</h3>
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<h3>Preparation of <A href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>&Delta;A (BBa_K103003)</a></h3>
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<h4>Michał K.</h4>
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<h4>Michał K., Piotr</h4>
<ol>
<ol>
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> and &Delta;A. </li></ol>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> and &Delta;A. </li><li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformation</a> with the above ligation. </li>
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<h3></h3>
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<h4>Piotr</h4>
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<ol><li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformation</a> with the above ligation. </li>
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<li>Plating on LB + ampicillin.</li>
<li>Plating on LB + ampicillin.</li>

Revision as of 20:46, 26 October 2008

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MutD5 testing

Piotr

Testing MutD5 ampicillin resistance - MutD5 has not spontaneous ampicillin resistance mutations.

'Hunter and prey' system tests: Competition tests

Weronika

Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)

  2. Bacteria centrifuged and frozen

Preparation of pSB1A3 carrying ΔA (BBa_K103003)

Michał K., Piotr

  1. Gel electrophoresis to estimate concentration of purified DNA from previous day.
  2. Ligation of isolated DNA fragments of pSB1A3 and ΔA.
  3. E. coli TOP10 transformation with the above ligation.
  4. Plating on LB + ampicillin.

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