Team:Mississippi State/Making Competent Cells

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#Cover with aluminum
#Cover with aluminum
#Put in 37C shaker for 2hr.
#Put in 37C shaker for 2hr.
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#[[Team:Mississippi State/Check cell density|Check cell density]]
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#Pour into centrifuge tubes.

Revision as of 20:37, 17 June 2008

Home The Team The Project Parts Submitted to the Registry Modeling Notebook
  • To make competent cells(XL1-Blue), do exactly as follows:
  1. Add 2ml LB Broth to Culture Tube.
  2. Add 1ul antibiotic for every 1ml LB(in this case, 2ul of Tetracycline).
  3. Get cell stock from -80C freezer.
  4. Scrape cells from top with tip of pipette.
  5. Add to LB Broth.
  6. Label (Culture name, date, iGEM)
  7. Put in 37C shaker overnight.
  8. Remove next morning.
  9. Get pipette gun from 1st drawer from left under lab bench closest to door.
  10. Get 10ml tube from lab bench opposite 1st lab bench.
  11. Add 25ml LB Broth to autoclaved flask(KEEP THE FOIL COVER!).
  12. Add 25ul Tet (in IGEM 2007 Box, -20C freezer)
  13. Add 250ul cells from overnight culture.
  14. Cover with aluminum
  15. Put in 37C shaker for 2hr.
  16. Check cell density
  17. Pour into centrifuge tubes.
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