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Team:Tokyo Tech/Construction
From 2008.igem.org
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For confirming pressure-response ability of pressure-inducible promoter, we | For confirming pressure-response ability of pressure-inducible promoter, we | ||
| - | experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet on pSB6([]) ,the other is ∆p on pSB6. | + | experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet on pSB6([http://partsregistry.org/Part:BBa_I739201 BBa_I739201]), the other is ∆p on pSB6. |
Ptet stands for promoter repressed by tetR protein. ∆p stands for promoter less. JM109 is used .And JM109 isn’t expression tetR. Therefore, we used Ptet as a positive control. | Ptet stands for promoter repressed by tetR protein. ∆p stands for promoter less. JM109 is used .And JM109 isn’t expression tetR. Therefore, we used Ptet as a positive control. | ||
Revision as of 05:56, 26 October 2008
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Construction
For confirming pressure-response ability of pressure-inducible promoter, we experimented under 30MPa pressure. We chose TetR promoter(Ptet) as a pressure-inducible promoter. we constructed two plasmids - one is Ptet on pSB6([http://partsregistry.org/Part:BBa_I739201 BBa_I739201]), the other is ∆p on pSB6.
Ptet stands for promoter repressed by tetR protein. ∆p stands for promoter less. JM109 is used .And JM109 isn’t expression tetR. Therefore, we used Ptet as a positive control.
