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Team:NTU-Singapore/Parts
From 2008.igem.org
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The FLx800™ Fluorescence Microplate Reader has a computer software KCJunior that was installed in a computer adjacent to the reader for collection and processing of data from our samples. | The FLx800™ Fluorescence Microplate Reader has a computer software KCJunior that was installed in a computer adjacent to the reader for collection and processing of data from our samples. | ||
| - | =Experiments= | + | =='''Experiments'''== |
| - | ==Characterization of Standard Biobrick via GFP Activity== | + | ===Characterization of Standard Biobrick via GFP Activity=== |
The objectives of the characterization experiment are to investigate the effects of concentration of inducer, temperature and time on the production of Green Fluorescence protein (GFP) by the Biobrick promoter part being experimented. | The objectives of the characterization experiment are to investigate the effects of concentration of inducer, temperature and time on the production of Green Fluorescence protein (GFP) by the Biobrick promoter part being experimented. | ||
A stronger fluorescence, as measured using NTU FLx800™ Fluorescence Microplate Reader will indicate more GFP proteins being synthesised. This also corresponds to higher promoter activity. | A stronger fluorescence, as measured using NTU FLx800™ Fluorescence Microplate Reader will indicate more GFP proteins being synthesised. This also corresponds to higher promoter activity. | ||
| - | + | Top 10 competent cells are transformed with plasmid carrying the following gene sequence: | |
[[Image:PLacI-GFP.JPG|center|thumb|400px|pLac-I GFP]] | [[Image:PLacI-GFP.JPG|center|thumb|400px|pLac-I GFP]] | ||
The cells are cultured and upon addition of the inducer, the reaction mixtures (with both cells and inducer) are fed into the Fluorescence Microplate Reader. This machine has a inbuilt software, KCJunior, which will translate the results into excellent data suitable for analysis. The samples’ fluorescence was measured for a period of 12 hours, at the temperatures of <br> | The cells are cultured and upon addition of the inducer, the reaction mixtures (with both cells and inducer) are fed into the Fluorescence Microplate Reader. This machine has a inbuilt software, KCJunior, which will translate the results into excellent data suitable for analysis. The samples’ fluorescence was measured for a period of 12 hours, at the temperatures of <br> | ||
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For more information please refer to [https://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP Characterization of pLacI-GFP] | For more information please refer to [https://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP Characterization of pLacI-GFP] | ||
| - | ==Characterization of New promoter pLsrA - YFP== | + | ===Characterization of New promoter pLsrA - YFP=== |
For more information please refer to [https://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_pLsrA-YFP Characterization of pLsrA-YFP] | For more information please refer to [https://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_pLsrA-YFP Characterization of pLsrA-YFP] | ||
Revision as of 07:51, 26 October 2008
|
Contents |
Characterization Introduction
The group proposed to discuss and investigate the working range of the parts and devices of our system
- The proposed variables to investigate are:
i) Concentration of activator
ii) Temperature
iii) Time
Characterization of Parts
We are interested in characterizing the following parts:
i) Lactose/IPTG sensitive Promoter pLac [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]
ii) Iron Sensitive Promoter pFe [http://partsregistry.org/wiki/index.php?title=Part:BBa_I716014 BBa_I716014]
iii) AI2 senstive Promoter pLsrA
The characterization is carried out by ligating the desired promoter with the Green Fluorescence protein: [http://partsregistry.org/Part:BBa_E0240 BBa_E02040]
- For the pLac BBa_R0010, it is already available in the form that we desired in the registry as [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]
- For the pLsrA promoter, the Yellow Fluorescence protein was used instead to differentiate between our two different systems.
The FLx800™ Fluorescence Microplate Reader
The FLx800™ Fluorescence Microplate Reader was available for our use courtesy of Division of Bio-engineering lab. It was purchased from BioTek and for more information about the reader, you can refer to [http://www.biotek.com/products/product_detail.php?pid=117 The FLx800™ Fluorescence Microplate Reader]
The FLx800™ Fluorescence Microplate Reader has a computer software KCJunior that was installed in a computer adjacent to the reader for collection and processing of data from our samples.
Experiments
Characterization of Standard Biobrick via GFP Activity
The objectives of the characterization experiment are to investigate the effects of concentration of inducer, temperature and time on the production of Green Fluorescence protein (GFP) by the Biobrick promoter part being experimented. A stronger fluorescence, as measured using NTU FLx800™ Fluorescence Microplate Reader will indicate more GFP proteins being synthesised. This also corresponds to higher promoter activity.
Top 10 competent cells are transformed with plasmid carrying the following gene sequence:
The cells are cultured and upon addition of the inducer, the reaction mixtures (with both cells and inducer) are fed into the Fluorescence Microplate Reader. This machine has a inbuilt software, KCJunior, which will translate the results into excellent data suitable for analysis. The samples’ fluorescence was measured for a period of 12 hours, at the temperatures of
i) 25°C
ii) 37°C
and iii) 42°C.
The readings were than plotted to produce a 3-dimentional graph for detail analysis.
For more information please refer to Characterization of pLacI-GFP
