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Team:Tokyo Tech/Construction
From 2008.igem.org
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Revision as of 09:27, 26 October 2008
| Home | Construction | Acrylic container | Development of promoter | Genetic toggle switch | Parts Submitted to the Registry | Result | The Team | Acknowledgements |
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Confirmatory experiment
For confirming pressure-response ability of pressure-inducible promoter, we experimented under 0.1MPa and 30MPa pressure. We chose TetR promoter(PtetR) as pressure-inducible promoter and we constructed two plasmids - one is PtetR-GFP on pSB6([http://partsregistry.org/Part:BBa_K121010 BBa_K121010]), the other is promoter less-GFP on pSB6([http://partsregistry.org/Part:BBa_K121013 BBa_K121013]) as a negative control.
The result was that PtetR activity under 30MPa pressure is 2.5 times stronger than PtetR activity under 0.1MPa pressure.
