Team:Warsaw/Calendar-Main/11 September 2008

From 2008.igem.org

(Difference between revisions)
Line 8: Line 8:
<p><ol><li>Inoculation of pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).</li>
<p><ol><li>Inoculation of pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).</li>
-
<li>Measurement of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> twice as more as <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> with protein Z_alpha etc..)</li></ol></p>
+
<li>Measurement of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> twice as more as <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> with protein Z_alpha etc..)!!!!</li></ol></p>

Revision as of 11:28, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Tests for ampicillin resistance given by protein added to medium

Piotr

  1. Inoculation of pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).
  2. Measurement of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (pACYC177+OmpA_A_omega twice as more as pACYC177+OmpA_omega with protein Z_alpha etc..)!!!!

We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/11_September_2008"