Team:Chiba/Calendar-Home/18 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Team:Communication)
Line 6: Line 6:
===Team:Communication===
===Team:Communication===
-
[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
+
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
-
*conditions
+
**transformants
-
#strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
+
#
-
#temparature<br>37℃ and 30℃
+
#
 +
#
 +
**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
 +
**temparature<br>37°C  and 30°C
===Team:Output===
===Team:Output===

Revision as of 12:39, 26 October 2008

>Home | Notebook

17 September 2008 <|> 19 September 2008

Laboratory work

Team:Communication

    • E.coli strain
      Senders:XL10GOLD
      Receiver:JW1908
    • temparature
      37°C and 30°C

Team:Output

SAP 1μL

->left for:

  1. 1 hour at 37 degrees
  2. 30 minutes at 65 degrees.

Zymoclean

->eluted with 25μL of NFW.

Ligation

  • vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
  • insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
  • Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
Sample No. Vector&insertVector
DNA(vector)(μL) 3.53.5
DNA(insert)(μL) 7.5-
Ligase(μL) 11
Ligase Buffer(μL) 44
dH2O(μL) 44
TOTAL(μL) 2020

transformation

  • [http://partsregistry.org/Part:BBa_J54103 BBa_J54103](RBS+GFP)
  • [http://partsregistry.org/Part:BBa_P2006 BBa_P2006]

Gel check

  • Ligation product(Venus YFP)
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
Sample No. each
DNA(μL) each
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/18_September_2008"