Team:Chiba/Calendar-Home/17 September 2008

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===Team:Communication===
===Team:Communication===
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
-
*transformants
+
**transformants
-
*[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
+
***[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
-
*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
+
***[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
-
*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
+
***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
-
*E.coli strain<br>Senders:JW1908<br>Receiver:JW1908<br>
+
**E.coli strain<br>Senders:JW1908<br>Receiver:JW1908<br>
-
*reaction temparature<br>37°C and 30°C  
+
**reaction temparature<br>37°C and 30°C  
 +
 
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
-
*transformants
+
**transformants
-
*[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
+
***[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
-
*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
+
***[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
-
*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
+
***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
-
*E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
+
**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
-
*reaction temparature<br>37°C and 30°C
+
**reaction temparature<br>37°C and 30°C
===Team:Output===
===Team:Output===

Revision as of 12:44, 26 October 2008

>Home | Notebook

16 September 2008 <|> 18 September 2008

Laboratory work

Team:Communication

  • time-delay test
    • transformants
      • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
      • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
      • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
    • E.coli strain
      Senders:JW1908
      Receiver:JW1908
    • reaction temparature
      37°C and 30°C


  • time-delay test
    • transformants
      • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
      • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
      • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
    • E.coli strain
      Senders:XL10GOLD
      Receiver:JW1908
    • reaction temparature
      37°C and 30°C

Team:Output

Transformed

  • [http://partsregistry.org/Part:BBa_I13500 BBa_I13500](RBS+GFP)
  • [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](Luxpr+LuxR)-->no colony

miniprepped

  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](Luxpr+LuxR+LuxR+GFP)
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)

Digestion

  • [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](ptetR+RBS)
  • [http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
  • Ligation product(ptet+LasR)
  • Ligation product(ptet+RhlR)
  • Ligation product(ptet+cinR)
Sample No. each
DNA template(μL) 25
EcoRⅠ(μL) 1
PstⅠ(μL) 1
BSA(x10)(μL) 4
EcoRⅠ Buffer(μL) 4
dH2O(μL) 5
TOTAL(μL) 40

-->Left at37 degrees for 1 hour.

-->Next day

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