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Team:Warsaw/Calendar-Main/3 July 2008
From 2008.igem.org
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<h4>Piotr, Weronika</h4> | <h4>Piotr, Weronika</h4> | ||
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| - | <li>There were blue and white colonies but no red or green in UV</li> | + | <li>There were blue and white colonies but no red or green fluorescence in UV</li> |
<li>Inoculation of liquid LB medium + Amp with white colonies</li> | <li>Inoculation of liquid LB medium + Amp with white colonies</li> | ||
Revision as of 17:21, 26 October 2008
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Change of the reporter from pZC320 with B-galactosidase to GFP or RFPPiotr, Weronika
Preparation of constructs on pET15b: OmpA_alpha and OmpA_omegaPawełColonies of each transformants from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin. Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, MarcinWe have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primer which causes frameshift and cloning of these fusions have to be repeated from the beginning.
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