Team:Heidelberg/Notebook/Sensing Group/Notebook/7thweek
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== Tuesday, 08/16/2008 == | == Tuesday, 08/16/2008 == | ||
+ | * Digestion of Fusion 1+2 with PstI | ||
+ | [[Image:HD 080916-F1 2 Digestion PstI.png|center|thumb|400px|Digestion of Fusion constructs with PstI. All, except Fusion 1 colony 2 of NcoI/NdeI cloning, seem to be positive]] | ||
+ | * F1+2 2,3 (KpnI), F1+2 1,3 (NdeI) sent to sequencing @ GATC | ||
+ | F2 NdeI #3, F1 NdeI #1, F1 KpnI #2 seem to be correct | ||
== Wednesday, 08/17/2008 == | == Wednesday, 08/17/2008 == |
Revision as of 17:30, 26 October 2008
Contents |
Tuesday, 08/16/2008
- Digestion of Fusion 1+2 with PstI
- F1+2 2,3 (KpnI), F1+2 1,3 (NdeI) sent to sequencing @ GATC
F2 NdeI #3, F1 NdeI #1, F1 KpnI #2 seem to be correct