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Team:Warsaw/Calendar-Main/7 August 2008
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<h3>Purification of proteins: Z-alpha and Z-omega</h3><h4>Piotr, Emilia</h4> | <h3>Purification of proteins: Z-alpha and Z-omega</h3><h4>Piotr, Emilia</h4> | ||
| - | <p><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BZ-alpha>pET15b+Z-alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2Bhis%2BZ%2Bomega>pET15b+Z-omega</a> inoculated for overnight culture (Rosetta strain)</p> | + | <p><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BZ-alpha>pET15b+Z-alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2Bhis%2BZ%2Bomega>pET15b+Z-omega</a> inoculated for overnight culture (Rosetta strain).</p> |
<h3>Preparing of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-deltaA>pACYC177+OmpA_omega_ΔA</a> construct</h3><h4>Michał K.</h4> | <h3>Preparing of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-deltaA>pACYC177+OmpA_omega_ΔA</a> construct</h3><h4>Michał K.</h4> | ||
Revision as of 18:10, 26 October 2008
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Purification of proteins: Z-alpha and Z-omegaPiotr, EmiliapET15b+Z-alpha and pET15b+Z-omega inoculated for overnight culture (Rosetta strain). Preparing of pACYC177+OmpA_omega_ΔA constructMichał K.
Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10)Michał L.Separate transformant colonies (Rosetta transformation from previous day) inoculated to liquid LB with kanamycin.  Fig. 1. Control SacI/HindIII digests of isolated plasmids1. Marker 2-9. digested plasmids pACYC177+OmpA_omega_deltaA
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