"
Team:Heidelberg/Notebook/Sensing Group/Notebook/8thweek
From 2008.igem.org
(Difference between revisions)
(→Monday, 09/22/2008) |
(→Tuesday, 09/23/2008) |
||
| Line 130: | Line 130: | ||
== Tuesday, 09/23/2008 == | == Tuesday, 09/23/2008 == | ||
| + | * Maxiprep of LuxP-Fusion constructs (F1(NdeI)_LuxP_pDK48, F1(KpnI)_LuxP_pDK48 and F2(NdeI)_LuxP_pDK48) | ||
| + | * Cloning of Fusion_LuxP in pBAD33 with BamHI/PstI | ||
| + | * digestions of Fusion constructs and pBAD33 as described in gel image descriptions | ||
| + | [[Image:HD 080923 Fusion-LuxP pBAD33 Digestion.png|left|thumb|200px|Digestion of Fusion constructs and pBAD33 with BamHI/PstI for subsequent cloning.]][[Image:HD 080923 Fusion-LuxP Digestion.png|left|thumb|400px|Digestion of Fusion constructs with NcoI and PstI to check for correct insert and Maxiprep digetions.]] | ||
| + | |||
| + | <br style="clear: both;"/> | ||
| + | |||
| + | * O/N culture of Fusion-LuxP-pDK48 in HCB33 and MG1655 in TB-Kan | ||
| + | * Transformation of Fusion-LuxP-pBAD33 constructs into DH5a | ||
== Wednesday, 09/24/2008 == | == Wednesday, 09/24/2008 == | ||
Revision as of 19:12, 26 October 2008
Contents |
Monday, 09/22/2008
- test digestion of F1_LuxP_NdeI (F1 cloned in pDK48 with NdeI), F2_LuxP_NdeI, F1_LuxP_KpnI (F1 cloned in pDK48 with KpnI) and F2_LuxP_KpnI (F2 cloned in pDK48 with KpnI) with NcoI
- Transformation of Fusion constructs (F1(NdeI)_LuxP_pDK48, F1(KpnI)_LuxP_pDK48 and F2(NdeI)_LuxP_pDK48) into MG1655 and HCB33 cells
- Preparation of cultures for Maxipreps of Fusion constructs
Tuesday, 09/23/2008
- Maxiprep of LuxP-Fusion constructs (F1(NdeI)_LuxP_pDK48, F1(KpnI)_LuxP_pDK48 and F2(NdeI)_LuxP_pDK48)
- Cloning of Fusion_LuxP in pBAD33 with BamHI/PstI
- digestions of Fusion constructs and pBAD33 as described in gel image descriptions
- O/N culture of Fusion-LuxP-pDK48 in HCB33 and MG1655 in TB-Kan
- Transformation of Fusion-LuxP-pBAD33 constructs into DH5a
