Team:Warsaw/Calendar-Main/19 August 2008
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<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> with NdeI and NotI (Orange buffer) and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylation with CIAP</a>.</li> | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> with NdeI and NotI (Orange buffer) and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylation with CIAP</a>.</li> | ||
<li> Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/19_August_2008#fig1">Fig. 1.</a>) and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of 6300 bp band. </li> </ol> | <li> Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/19_August_2008#fig1">Fig. 1.</a>) and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of 6300 bp band. </li> </ol> | ||
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+ | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/4/4b/PKS%2BApGApET%2Balfa.JPG"></a> | ||
+ | <var><b>Fig. 1.</b>Digest product - pGeneart+Z (lanes 1-3), pKS+A (4-6), pET15b+OmpA-A (8-10). Lane 7 - GeneRuler™ DNA Ladder Mix</var> | ||
+ | </p> | ||
<h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4> | <h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4> |
Revision as of 20:23, 26 October 2008
Tests for ampicillin resistance given by protein added to mediumPiotrInoculation of TOP10 carrying pACYC177+OmpA_A_alpha, pACYC177+OmpA_ΔA_alpha, pACYC177+OmpA_ΔA_omega, pACYC177+OmpA_A_omega, pACYC177+OmpA_omega_ΔA, pACYC177+OmpA_alpha, pACYC177+OmpA_omega and pACYC177+OmpA_omega_ΔA_alpha to LB with inductor (0.25 mM IPTG) and kanamycin. Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.
Cloning of protein A DNA to pET15b+OmpA-alpha plasmidAntoni
Cloning of protein A DNA to GeneArt plasmidAntoni
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