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Team:Warsaw/Calendar-Main/14 August 2008
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/35/August_14_th_2.jpg" width=250 /></a><var><b>Fig. 1.</b> Fractions from purifying Z-alpha and Z-omega protein fusions.<br> | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/35/August_14_th_2.jpg" width=250 /></a><var><b>Fig. 1.</b> Fractions from purifying Z-alpha and Z-omega protein fusions.<br> | ||
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| - | 1. | + | 1. Purified Z-omega<br> |
| - | 2. | + | 2. Supernatant Z-alpha after sonication<br> |
| - | 3. | + | 3. Elution of Z-alpha using ddH2o<br> |
| - | 4. | + | 4. Purified Z-alpha<br> |
5. Marker<br></var> | 5. Marker<br></var> | ||
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1. Marker<br> | 1. Marker<br> | ||
| - | 2. | + | 2. Sonicate Z-omega after induction<br> |
| - | 3. | + | 3. Pellet Z-omega after induction<br></var> |
Latest revision as of 15:40, 28 October 2008
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Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omegaMichał K.
Purification of proteins: Z-alpha and Z-omegaPiotrElectrophoresis in polyacrylamide gel (12 %) of sonicate and preliminarily purified protein fusions: Z-alpha and Z-omega (Fig. 1. and Fig. 2.)  Fig. 1. Fractions from purifying Z-alpha and Z-omega protein fusions.1. Purified Z-omega 2. Supernatant Z-alpha after sonication 3. Elution of Z-alpha using ddH2o 4. Purified Z-alpha 5. Marker  Fig. 2. Sonicate and pellet from Z-omega induction1. Marker 2. Sonicate Z-omega after induction 3. Pellet Z-omega after induction
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