Template:Team:UC Berkeley/Notebook/AL construction

From 2008.igem.org

(Difference between revisions)
(→pBca1256-K112319)
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CGttaGGATCCcttctatccgctcacgtatc
CGttaGGATCCcttctatccgctcacgtatc
</pre>
</pre>
 +
==pBca1256-K112319==
==pBca1256-K112319==
<pre>
<pre>
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CGttaGGATCCgtaccaatctccagtcctactaccag
CGttaGGATCCgtaccaatctccagtcctactaccag
</pre>
</pre>
 +
 +
==pBjh1601AC-K112321==
 +
<pre>
 +
PCR JH088F/JHO88R on Salmonella L2.    (435bp, EcoRI/BamHI/DpnI)
 +
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
 +
Product is pBjh1601AC-K112321
 +
-----------------------------------------------
 +
JH088F  Forward biobricking of {H-NS!} 
 +
cgataGAATTCatgAGATCTATGAGCGAAGCACTTAAAATTC
 +
JH088R  Reverse biobricking of {H-NS!} 
 +
cgttaGGATCCTTATTGCTTGATCAGGAAATCG
 +
</pre>
 +
 +
==pBjh1601CK-K112322==
 +
PCR JH089F/JH089R on Salmonella L2.    (379 bp, EcoRI/BamHI/DpnI)
 +
Sub into pBjh1601CK            (EcoRI/BamHI, 3134+910, L)
 +
Product is pBjh1601CK-K112322
 +
-----------------------------------------------
 +
JH089F  Forward biobricking of {Pdps} 
 +
cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc
 +
JH089R  Reverse biobricking of {Pdps} 
 +
cgttaGGATCCaatctcatatcctcttgatg
 +
 +
==pBCa1601AC-K112323==
 +
PCR ca998/JH090R on pBjh1601AC-K112321-2    (445 bp, EcoRI/BamHI)
 +
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
 +
Product is pBjh1601AC-K112323
 +
-----------------------------------------------
 +
ca998  Forward biobricking of {H-NS!} Salmonella 
 +
gtatcacgaggcagaatttcag
 +
JH090R  Reverse biobricking of {H-NS!} Salmonella 
 +
cgttaGGATCCTTATTcCTTGATCAGG

Revision as of 18:36, 9 July 2008

Contents

pBca1256-K112300

PCR al0001/al0002 on pSB3C6-Bjh1501     (508bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112300
-----------------------------------------------
al0001	Forward Biobricking of lambda lysozyme with start and stop codons
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of lambda lysozyme with start and stop codons
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112301

PCR al0003/al0002 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112301
-----------------------------------------------
al0003	Forward Biobricking of Lambda Lysozyme with stop codon and no start codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112302

PCR al0001/al0004 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112302
-----------------------------------------------
al0001	Forward Biobricking of lambda lysozyme with start codon and no stop codon
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0004	Reverse Biobricking of lambda lysozyme with start codon and no stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc

pBca1256-K112303

PCR al0003/al0004 on pSB3C6-Bjh1501     (502bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112303
-----------------------------------------------
al0003	Forward Biobricking of lambda lysozyme with no start/stop codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0004	Reverse Biobricking of lambda lysozyme with no start/stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc

pBca1256-K112304

PCR al0005/al0002 on pSB3C6-Bjh1501     (512bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112304
-----------------------------------------------
al0005	Forward Biobricking of lambda lysozyme with spacer
CCATAGAATTCatgAGATCTgaagatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of lambda lysozyme with spacer
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112305

PCR al0006/al0002 on pSB3C6-Bjh1501     (527bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112305
-----------------------------------------------
al0006	Forward Biobricking of lambda lysozyme with rbs site
CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg
al0002	Reverse Biobricking of lambda lysozyme with rbs site
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112306

PCR al0007/al0008 on pSB3C6-Bjh1502     (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112306
-----------------------------------------------
al0007	Forward Biobricking of lambda holin with start and stop codons
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0008	Reverse Biobricking of lambda holin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112307

PCR al0009/al0008 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112307
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with stop codon and no start codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0008	Reverse Biobricking of lambda holin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112308

PCR al0007/al0010 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112308
-----------------------------------------------
al0007	Forward Biobricking of lambda holin with start and no stop codon
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0010	Reverse Biobricking of lambda holin wtih start and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112309

PCR al0009/al0010 on pSB3C6-Bjh1502     (343bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112309
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with no start and stop codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0010	Reverse Biobricking of lambda holin with no start and stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112310

PCR al0011/al0008 on pSB3C6-Bjh1502     (353bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112310
-----------------------------------------------
al0011	Forward Biobricking of lambda holin with spacer
CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg
al0008	Reverse Biobricking of lambda holin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112311

PCR al0012/al0008 on pSB3C6-Bjh1502     (369bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112311
-----------------------------------------------
al0012	Forward Biobricking of lambda holin with rbs site
CCATAGAATTCatgAGATCTattgggggtaagacCtgaag
al0008	Reverse Biobricking of lambda holin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112312

PCR al0013/al0008 on pSB3C6-Bjh1503     (355bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112312
-----------------------------------------------
al0013	Forward Biobricking of lambda antiholin with start and stop codons
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112313

PCR al0014/al0008 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112313
-----------------------------------------------
al0014	Forward Biobricking of lambda antiholin with stop codon and no start codon
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0008	Reverse Biobricking of lambda antiholin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112314

PCR al0013/al0010 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112314
-----------------------------------------------
al0013	Forward Biobricking of lambda antiholin with start codon and no stop codon
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0010	Reverse Biobricking of lambda antiholin with start codon and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112315

PCR al0014/al0010 on pSB3C6-Bjh1503     (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112315
-----------------------------------------------
al0014	Forward Biobricking of lambda antiholin with no start and stop codons
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0010	Reverse Biobricking of lambda antiholin with no start and stop codons
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112316

PCR al0015/al0008 on pSB3C6-Bjh1503     (359bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112316
-----------------------------------------------
al0015	Forward Biobricking of lambda antiholin with spacer
CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112317

PCR al0016/al0008 on pSB3C6-Bjh1503     (375bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112317
-----------------------------------------------
al0016	Forward Biobricking of lambda antiholin with rbs site
CCATAGAATTCatgAGATCTccccttattgggggtaagacatg
al0008	Reverse Biobricking of lambda antiholin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112318

PCR al0017/al0018 on MG1655     (467bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112318
-----------------------------------------------
al0017	Forward Biobricking of bolA promoter
CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc
al0018	Reverse Biobricking of bolA promoter
CGttaGGATCCcttctatccgctcacgtatc

pBca1256-K112319

PCR al0019/al0020 on MG1655     (465bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112319
-----------------------------------------------
al0019	Forward Biobricking of ftsQ promoter
CCATAGAATTCatgAGATCTttgattgaaaaatggctaagtgggccgg
al0020	Reverse Biobricking of ftsQ promoter
CGttaGGATCCcctcttcttcgctgtttcgc
al0023 Forward Removing of EcoRI
ggtagtacgaattGtggaactggcg
al0024 Reverse Removing of EcoRI
cgccagttccaCaattcgtactacc

pBca1256-K112320

PCR al0021/al0022 on MG1655     (804bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112320
-----------------------------------------------
al0021	Forward Biobricking of ftsAZ promoter
CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg
al0022	Reverse Biobricking of ftsAZ promoter
CGttaGGATCCgtaccaatctccagtcctactaccag

pBjh1601AC-K112321

PCR JH088F/JHO88R on Salmonella L2.     (435bp, EcoRI/BamHI/DpnI)
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
Product is pBjh1601AC-K112321
-----------------------------------------------
JH088F   Forward biobricking of {H-NS!}   
cgataGAATTCatgAGATCTATGAGCGAAGCACTTAAAATTC
JH088R   Reverse biobricking of {H-NS!}   
cgttaGGATCCTTATTGCTTGATCAGGAAATCG

pBjh1601CK-K112322

PCR JH089F/JH089R on Salmonella L2. (379 bp, EcoRI/BamHI/DpnI) Sub into pBjh1601CK (EcoRI/BamHI, 3134+910, L) Product is pBjh1601CK-K112322

JH089F Forward biobricking of {Pdps} cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc JH089R Reverse biobricking of {Pdps} cgttaGGATCCaatctcatatcctcttgatg

pBCa1601AC-K112323

PCR ca998/JH090R on pBjh1601AC-K112321-2 (445 bp, EcoRI/BamHI) Sub into pBjh1601AC (EcoRI/BamHI, 3195+910, L) Product is pBjh1601AC-K112323

ca998 Forward biobricking of {H-NS!} Salmonella gtatcacgaggcagaatttcag JH090R Reverse biobricking of {H-NS!} Salmonella cgttaGGATCCTTATTcCTTGATCAGG

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