EPF-Lausanne/15 September 2008

(Difference between revisions)

Revision as of 10:02, 27 October 2008

Before sending the plasmids for sequencing we test our primers by PCR.

PCR on AB with:

PCR on CD with:

Results:

--> image of PCR/gel page 102

All results were as expected except for AB lane 3, i.e. AB2seqFW, LacImRV

A new PCR is done for these primers, where the annealing temperature is reduced to 53°C. This turns out to work.

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+==Sequencing==
+Before sending the plasmids for sequencing we test our primers by PCR.
+PCR on AB with:
+*pSB1A2 FW, I1466RV
+*AB1seqFW, I1466RV
+*AB2seqFW,LacImRV
+*R0071FW,pSB1A2RV
+PCR on CD with:
+*pSB1A2FW, C0040RV
+*CD1FW, C0040RV
+*R0040FW, BB3
+*R0040FW, pSB1A2RV
+'''Results:'''
+--> image of PCR/gel page 102
+All results were as expected except for AB lane 3, i.e. AB2seqFW, LacImRV
+A new PCR is done for these primers, where the annealing temperature is reduced to 53°C.
+This turns out to work.