Team:Warsaw/Calendar-Main/18 September 2008
From 2008.igem.org
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<li> Gel electrophoresis of digested plasmid and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> - 2200 bp).</li></ol> | <li> Gel electrophoresis of digested plasmid and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (<a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> - 2200 bp).</li></ol> | ||
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+ | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/d/d0/Go_17_09.jpg" width=300/></a><var><b>Fig. 2. PCR to obtain inserts</b><br> | ||
+ | 1. Marker<br> | ||
+ | 2. deltaA<br> | ||
+ | 3. omega<br> | ||
+ | 4. ompA_omega<br></var> | ||
+ | </p> | ||
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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103009>linker_alpha (BBa_K103009)</a></h3> | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103009>linker_alpha (BBa_K103009)</a></h3> | ||
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</ol> | </ol> | ||
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1. Marker<br> | 1. Marker<br> | ||
2. deltaA<br> | 2. deltaA<br> | ||
- | 3 omega<br> | + | 3. omega<br> |
4. ompA_omega<br></var> | 4. ompA_omega<br></var> | ||
</p> | </p> | ||
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1. Marker<br> | 1. Marker<br> | ||
2. deltaA<br> | 2. deltaA<br> | ||
- | 3 omega<br> | + | 3. omega<br> |
4. ompA_omega<br></var> | 4. ompA_omega<br></var> | ||
</p> | </p> | ||
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1. Marker<br> | 1. Marker<br> | ||
2. deltaA<br> | 2. deltaA<br> | ||
- | 3 omega<br> | + | 3. omega<br> |
4. ompA_omega<br></var> | 4. ompA_omega<br></var> | ||
Revision as of 13:12, 27 October 2008
'Hunter and prey' system tests: Competition testsWeronikaInoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_Z_omega, pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_alpha + induction using 0.25mM iPTG MutD5 testingPiotrInoculation of mutD5 into 100 ml of LB and rendering them chemocompetent. Electroporation with pACYC177+OmpA_Z_alpha, pACYC177+OmpA_Z_omega, pACYC177+OmpA_A_omega and pACYC177+OmpA_omega_ΔA. Optimisation of primers for preparation of partsMichał K.
1. Marker 2. 55 °C linker_alpha 3. 60 °C linker_alpha 4. 65 °C linker_alpha 5. 70 °C linker_alpha Preparation of ΔA (BBa_K103003)Michał K.
Preparation of linker_alpha (BBa_K103009)Michał K.
Preparation of linker_omega (BBa_K103013)Michał K.
Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of OmpA-linker (BBa_K103006)Michał K.
1. Marker 2. deltaA 3. omega 4. ompA_omega Fig. 3. PCR to obtain linker_alpha 1. Marker 2. linker_alpha Fig. 4. Control SacI/NdeI digest of pET15b_OmpA_omega 1. Marker 2. digested pSB1A3 Fig. 5. Control EcoRI/BcuI digest of pSB1A3 1. Marker 2. digested pSB1A3
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