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EPF-Lausanne/20 October 2008
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| + | |||
| + | ==Cloning== | ||
| + | |||
| + | *Cloning of BC1 into AB | ||
| + | **Using the simplified ligation protocol. | ||
| + | |||
| + | *Cloning of CD into LuxI+ (LuxI with RBS and Term.) | ||
| + | **Here a modified version of the ligation protocol was used, because the insert is quite big (over 2kb). | ||
| + | **Reaction mix: | ||
| + | ***3μl vector | ||
| + | ***5.5μl insert | ||
| + | ***1μl Ligase Buffer with ATP | ||
| + | ***0.5μl T4 Ligase | ||
| + | |||
| + | => Cloning worked. | ||
Revision as of 19:50, 28 October 2008
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Cloning
- Cloning of BC1 into AB
- Using the simplified ligation protocol.
- Cloning of CD into LuxI+ (LuxI with RBS and Term.)
- Here a modified version of the ligation protocol was used, because the insert is quite big (over 2kb).
- Reaction mix:
- 3μl vector
- 5.5μl insert
- 1μl Ligase Buffer with ATP
- 0.5μl T4 Ligase
=> Cloning worked.
