EPF-Lausanne/27 October 2008

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==Cloning==
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*Cloning of CD+ into AB+
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First we tried to use AB+ as the insert, but then realized that AB+ and its vector both had almost equal sizes (around 2kb). It was therefore not possible to separate them using gel purification.
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Fortunately, CD+ has 2.3kb, so we insert CD+ into AB+.
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Again we use the modified ligation protocol:
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**Protocol:
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***

Revision as of 19:55, 28 October 2008

<<Previous - Back to Notebook

Cloning

  • Cloning of CD+ into AB+

First we tried to use AB+ as the insert, but then realized that AB+ and its vector both had almost equal sizes (around 2kb). It was therefore not possible to separate them using gel purification.

Fortunately, CD+ has 2.3kb, so we insert CD+ into AB+.

Again we use the modified ligation protocol:

    • Protocol:
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