Team:Bay Area RSI/Project

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Revision as of 19:12, 27 October 2008

This is a template page. READ THESE INSTRUCTIONS.

You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.

You MUST have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace.

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)	Your team picture
	Team Example 2

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)

Project Overview

Every year over 1.2 million people suffer myocardial infarction. The resulting heart damage requires new approaches for effective repair. Stem cell therapies provide hope. However none of the stem cell therapies currently in clinical trials addresses the need for efficient stem cell targeting to cardiac tissue or the need to replace efficiently dead tissue with new cardiomyocytes. To address these problems, we have built several genetic circuits that work sequentially to repair the heart. First, we have built an inducible differentiation circuit that closely resembles the endogenous differentiation pathway, to program cells to become cardiomyocytes. Second, we have built circuits that use the extracellular domains of chimeric proteins to target cells to damaged cardiac tissue. Upon binding, novel receptor-coupled intein-mediated signaling domains activate effector genes that then aid in integration, inhibition of cell death, and the alteration of the tissue microenvironment.

'Targeting Infarcted Cardiac Tissue'

C-Reactive Protein Receptor Intein Mediated Signaling Circuit

overview signaling picture construct image

FCGamma Receptor Binds Immobilised CRP on Damaged Cardiomyocytes In Vitro

description picture picture exp

CRP activates GFP signaling upon FCGamma Binding

picture picture exp

Effectors To Aid in integration, anti-apoptosis, and the alteration of the tissue microenvironment

Overview Construct image pics from slides

@@ Line 55: / Line 55: @@
 === C-Reactive Protein Receptor Intein Mediated Signaling Circuit ===
+[[Image:CRP Signaling System diagram.jpg]]
 overview
 signaling picture
 construct image
 === FCGamma Receptor Binds Immobilised CRP on Damaged Cardiomyocytes In Vitro ===