Team:Bay Area RSI/Project

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(Project Details)
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=== C-Reactive Protein Receptor Intein Mediated Signaling Circuit ===
=== C-Reactive Protein Receptor Intein Mediated Signaling Circuit ===
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[[Image:CRP Signaling System diagram.jpg]]
overview
overview
signaling picture
signaling picture
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construct image  
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=== FCGamma Receptor Binds Immobilised CRP on Damaged Cardiomyocytes In Vitro ===
=== FCGamma Receptor Binds Immobilised CRP on Damaged Cardiomyocytes In Vitro ===

Revision as of 19:12, 27 October 2008


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
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Contents

Project Overview

Every year over 1.2 million people suffer myocardial infarction. The resulting heart damage requires new approaches for effective repair. Stem cell therapies provide hope. However none of the stem cell therapies currently in clinical trials addresses the need for efficient stem cell targeting to cardiac tissue or the need to replace efficiently dead tissue with new cardiomyocytes. To address these problems, we have built several genetic circuits that work sequentially to repair the heart. First, we have built an inducible differentiation circuit that closely resembles the endogenous differentiation pathway, to program cells to become cardiomyocytes. Second, we have built circuits that use the extracellular domains of chimeric proteins to target cells to damaged cardiac tissue. Upon binding, novel receptor-coupled intein-mediated signaling domains activate effector genes that then aid in integration, inhibition of cell death, and the alteration of the tissue microenvironment.

'Targeting Infarcted Cardiac Tissue'

C-Reactive Protein Receptor Intein Mediated Signaling Circuit

CRP Signaling System diagram.jpg overview signaling picture construct image

FCGamma Receptor Binds Immobilised CRP on Damaged Cardiomyocytes In Vitro

description picture picture exp

CRP activates GFP signaling upon FCGamma Binding

picture picture exp

Effectors To Aid in integration, anti-apoptosis, and the alteration of the tissue microenvironment

Overview Construct image pics from slides


Future Directions

Results