Team:Bay Area RSI/Project

From 2008.igem.org

(Difference between revisions)
(→'Targeting Infarcted Cardiac Tissue')
(→Targeting Infarcted Cardiac Tissue)
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[[Image:CRP Signaling System diagram.jpg]]
[[Image:CRP Signaling System diagram.jpg]]
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overview
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Continuation from last year
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signaling picture
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Circuit overview
construct image
construct image
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picture
picture
picture exp
picture exp
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=== Generation of Clonal lines of FCGamma+ rat H9C2 Cardiomyocytes  ===
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pic + explanation
=== Effectors To Aid in integration, anti-apoptosis, and the alteration of the tissue microenvironment ===
=== Effectors To Aid in integration, anti-apoptosis, and the alteration of the tissue microenvironment ===
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pics from slides  
pics from slides  
 +
=== Differentiation Circuit Concerns  ===
=== Future Directions  ===
=== Future Directions  ===
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Sensor sensitivity-> , avidity, affinity
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More universal receptor --> 2nd gen SCFV to target other tissue
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 +
== '''''Cardiomyocyte Differentiation Circuit'''''==
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endogenous pathways
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pathway overview
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construct image + explanation
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data
 +
 +
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=== Differentiation Circuit Concerns  ===
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CA, efficiency, transdiff,
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=== Future Directions  ===
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inducible, non-SC progenitor, native cardiofiobroblast
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 +
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== '''''Cardiomyocyte Differentiation Circuit'''''==
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Therpeautic approach with combined circuitry
 +
use in tandem, synergistic application
== Results ==
== Results ==

Revision as of 19:31, 27 October 2008


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Contents

Project Overview

Every year over 1.2 million people suffer myocardial infarction. The resulting heart damage requires new approaches for effective repair. Stem cell therapies provide hope. However none of the stem cell therapies currently in clinical trials addresses the need for efficient stem cell targeting to cardiac tissue or the need to replace efficiently dead tissue with new cardiomyocytes. To address these problems, we have built several genetic circuits that work sequentially to repair the heart. First, we have built an inducible differentiation circuit that closely resembles the endogenous differentiation pathway, to program cells to become cardiomyocytes. Second, we have built circuits that use the extracellular domains of chimeric proteins to target cells to damaged cardiac tissue. Upon binding, novel receptor-coupled intein-mediated signaling domains activate effector genes that then aid in integration, inhibition of cell death, and the alteration of the tissue microenvironment.

Targeting Infarcted Cardiac Tissue

C-Reactive Protein Receptor Intein Mediated Signaling Circuit

CRP Signaling System diagram.jpg Continuation from last year Circuit overview construct image

FCGamma Receptor Binds Immobilised CRP on Damaged Cardiomyocytes In Vitro

description picture picture exp

CRP activates GFP signaling upon FCGamma Binding

picture picture exp

Generation of Clonal lines of FCGamma+ rat H9C2 Cardiomyocytes

pic + explanation

Effectors To Aid in integration, anti-apoptosis, and the alteration of the tissue microenvironment

Overview Construct image pics from slides

Differentiation Circuit Concerns

Future Directions

Sensor sensitivity-> , avidity, affinity More universal receptor --> 2nd gen SCFV to target other tissue

Cardiomyocyte Differentiation Circuit

endogenous pathways pathway overview construct image + explanation data


Differentiation Circuit Concerns

CA, efficiency, transdiff,

Future Directions

inducible, non-SC progenitor, native cardiofiobroblast


Cardiomyocyte Differentiation Circuit

Therpeautic approach with combined circuitry use in tandem, synergistic application

Results

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